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<p>A novel analytical method was designed and developed that exhibited ultraviolet–visible (UV–Vis), fluorescence (FL), and resonance Rayleigh scattering (RRS) signals for straightforward and comprehensive determination of monoamine oxidase B&#xa0;(MAO-B) using polyethylenimine-functionalized silver nanoparticles (PEI-Ag NPs). Through a facile one-step experiment, and&#xa0;NaOH assisted, in an aqueous solution of 100 ℃ for 40&#xa0;min PEI reacted with AgNO<sub>3</sub> to generate PEI-Ag NPs with a yellow color and weak blue fluorescence. Interestingly, phenylacetaldehyde (PAA), a specific product of MAO-B, causes significant enhancement of the three optical signals of UV–Vis, FL, and RRS. A triple-signal readout sensing system was designed and constructed for MAO-B concentration assay. The limit of detection (LOD) was 0.42 (UV–Vis), 3.49 (FL), and 1.97 (RRS) <i>μ</i>g/mL. AND logic gate analysis was applied to enhance its accurate identification. Eventually, the feasibility of the method was assessed by measuring MAO-B amount in human serum samples with satisfying&#xa0;recoveries. The method provided a new concept&#xa0;for the clinical detection of MAO-B.</p> Graphical abstract <p></p>

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MAO-B-triggered reaction for an optical triple-signal assay and AND logic gate application based on PEI-functionalized silver nanoparticles

  • 期刊论文

  • 发表日期：

    2025年1月21日

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  • Xin-Ru Zhang,
  • Tian Chai,
  • Xiang-Lei Chang,
  • Ying Zhang,
  • Cheng-Bo Wang,
  • Song Liu,
  • Yves Iradukunda,
  • Xian-Yi Shang,
  • Yong-Hong Cao,
  • Wei-Feng Wang,
  • Jun-Li Yang,
  • Yin Qiang

摘要

A novel analytical method was designed and developed that exhibited ultraviolet–visible (UV–Vis), fluorescence (FL), and resonance Rayleigh scattering (RRS) signals for straightforward and comprehensive determination of monoamine oxidase B (MAO-B) using polyethylenimine-functionalized silver nanoparticles (PEI-Ag NPs). Through a facile one-step experiment, and NaOH assisted, in an aqueous solution of 100 ℃ for 40 min PEI reacted with AgNO3 to generate PEI-Ag NPs with a yellow color and weak blue fluorescence. Interestingly, phenylacetaldehyde (PAA), a specific product of MAO-B, causes significant enhancement of the three optical signals of UV–Vis, FL, and RRS. A triple-signal readout sensing system was designed and constructed for MAO-B concentration assay. The limit of detection (LOD) was 0.42 (UV–Vis), 3.49 (FL), and 1.97 (RRS) μg/mL. AND logic gate analysis was applied to enhance its accurate identification. Eventually, the feasibility of the method was assessed by measuring MAO-B amount in human serum samples with satisfying recoveries. The method provided a new concept for the clinical detection of MAO-B.

Graphical abstract