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Abstract <p> A&#xa0;nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma&#xa0;is proposed. Anti-CD63 antibody-modified biochips were prepared&#xa0;for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×10<sup>4</sup> particles/mL and a linear range from 10<sup>4</sup> to 10<sup>8</sup> particles/mL. By determination of&#xa0;exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer’s desease (AD) and healthy mice. The&#xa0;SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.</p> Graphical abstract <p></p>

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A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma

  • 期刊论文

  • 发表日期：

    2025年1月3日

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  • Junli Zhang,
  • Yanyan Zhao,
  • Haiying Hu,
  • Min Lv,
  • Hongling Zhang

摘要

Abstract

A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×104 particles/mL and a linear range from 104 to 108 particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer’s desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.

Graphical abstract