<p>Mining-induced seismicity (MIS) poses significant threats to public safety, disrupts the continuity of normal mining operations, degrades the ecological environment, and may even lead to casualties. Effective prevention and control of MIS has therefore become an urgent and critical challenge in mining engineering. Recent field experience gained from an industrial-scale trial of overburden isolated grout injection conducted at the Shilawusu coal mine in Inner Mongolia is summarized in this study. This trial was carried out to mitigate MIS in the Ultra-thick Cretaceous Strata (UTCS). The process flow and construction requirements of this technique are described in detail. Particular emphasis is placed on the critical roles of the grouting horizon, grouting borehole layout, grouting termination conditions, and grouting timing in ensuring the effectiveness of grouting operations. Based on in situ measurement data as well as mining and geological conditions, a detailed procedure for determining these parameters is presented. In addition, emergency response measures were promptly implemented to address the interference of extreme external environmental conditions on the grouting operation. As a result, this trial achieved satisfactory effects and benefits, including a reduced surface subsidence rate, the prevention of the recurrence of MIS in the UTCS, and the realization of zero coal gangue discharge during mining. Importantly, the protection of groundwater resources within the mining area was also achieved. This study aims to provide a practical reference framework by sharing the valuable experience gained from a successful MIS mitigation case, for mining areas with similar geological and mining conditions affected by MIS.</p>