Scholarlink

<p>Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disorder characterized by axonal degeneration, demyelination, and inflammation. However, current imaging and clinical biomarkers do not yet enable effective patient characterization and prediction. Here, we used large extracellular vesicles (LEVs) from cerebrospinal fluid (CSF) samples to analyze their potential for biomarker applications. LEVs were isolated from patients with MS and healthy subjects using an ultracentrifuge. LEVs were characterized by Western blotting, scanning electron microscopy, and dynamic light scattering techniques. The protein levels of IL-17 and TNF-ɑ in LEV samples were measured by ELISA. The characterization methods confirmed the successful isolation of LEVs in both groups of subjects. Our results showed that the protein levels of either IL-17 or TNF-ɑ were high in LEV samples of patients with MS compared to healthy subjects (<i>P</i> &lt; 0.05). ROC curve analysis showed that the area under the curve (AUC) was significant for IL-17 and TNF-α. Data indicated increased protein levels of IL-17 and TNF-α in LEVs from the patient group, which may propose a biomarker for investigation of inflammation and neurodegenerative sign in MS.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Extracellular vesicles derived from cerebrospinal fluid of patients with multiple sclerosis contain inflammatory cytokines: a possible biomarker potential

  • 期刊论文

  • 发表日期：

    2026年2月6日

查看全文
  • Razieh Momen-Mesgin,
  • Vahid Nejati,
  • Mehdi Hassanpour,
  • Jafar Rezaie

摘要

Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disorder characterized by axonal degeneration, demyelination, and inflammation. However, current imaging and clinical biomarkers do not yet enable effective patient characterization and prediction. Here, we used large extracellular vesicles (LEVs) from cerebrospinal fluid (CSF) samples to analyze their potential for biomarker applications. LEVs were isolated from patients with MS and healthy subjects using an ultracentrifuge. LEVs were characterized by Western blotting, scanning electron microscopy, and dynamic light scattering techniques. The protein levels of IL-17 and TNF-ɑ in LEV samples were measured by ELISA. The characterization methods confirmed the successful isolation of LEVs in both groups of subjects. Our results showed that the protein levels of either IL-17 or TNF-ɑ were high in LEV samples of patients with MS compared to healthy subjects (P < 0.05). ROC curve analysis showed that the area under the curve (AUC) was significant for IL-17 and TNF-α. Data indicated increased protein levels of IL-17 and TNF-α in LEVs from the patient group, which may propose a biomarker for investigation of inflammation and neurodegenerative sign in MS.