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<p> Canine blood transfusions are vital life-saving therapeutics in veterinary clinical practice, but the choice of anticoagulant solution significantly affects stored blood stability and quality. This study evaluated hematological and biochemical parameters of Nigerian indigenous dog blood stored in citrate–phosphate-dextrose-adenine (CPDA-1) and citrate–phosphate-dextrose-saline-adenine-glucose-mannitol (CPD-SAGM) solutions. Twenty-four unit bags of whole blood donated from dogs and preserved in&#xa0;CPDA-1 (12 bags) and CPD-SAGM (12) were stored at 4 °C for 6&#xa0;weeks. Each week, 3&#xa0;ml of the blood was drawn aseptically to measure hematological and serum biochemical parameters. Significant changes (<i>P</i> &lt; 0.05) in packed cell volume (week 4), mean corpuscular volume (week 1 and 4), and total white blood cell count (week 3) were observed in CPDA-1 blood compared to CPD-SAGM. Glucose levels were higher in CPD-SAGM in weeks 5 and 6, while lactate dehydrogenase was significantly (<i>P</i> &lt; 0.05) decreased. Aspartate aminotransferase showed significant increase in the CPDA-1 (<i>P</i> &gt; 0.05) in weeks 7, 5, and 6. No significant difference was observed in the potassium concentration but sodium levels were higher in weeks 1 to 6 in the CPDA-1 blood bags. The result shows that hematological and serum biochemical changes were present in blood stored in CPDA-1 bags early compared to CPD-SAGM anticoagulant solution. In addition, blood stored in CPDA-1 bags may not maintain the cells viability for more than 35&#xa0;days while blood can be stored for up to 42&#xa0;days in the CPD-SAGM blood bags. Based on the findings, CPD-SAGM appeared to be a better blood storage solution than CPDA-1 in veterinary medicine due to it better glucose preservation, lower sodium, and lactate dehydrogenase concentrations which suggest reduced cellular damage.</p>

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Enhancing canine blood storage: hematological and biochemical stability of Nigerian indigenous dogs’ blood in citrate–phosphate-dextrose-adenine and citrate–phosphate-dextrose-saline-adenine-glucose-mannitol solutions

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Uduak Joshua,
  • Ebenizer Olaoluwa Abidioye,
  • Tagang Aluwong,
  • Dahiru Sani

摘要

Canine blood transfusions are vital life-saving therapeutics in veterinary clinical practice, but the choice of anticoagulant solution significantly affects stored blood stability and quality. This study evaluated hematological and biochemical parameters of Nigerian indigenous dog blood stored in citrate–phosphate-dextrose-adenine (CPDA-1) and citrate–phosphate-dextrose-saline-adenine-glucose-mannitol (CPD-SAGM) solutions. Twenty-four unit bags of whole blood donated from dogs and preserved in CPDA-1 (12 bags) and CPD-SAGM (12) were stored at 4 °C for 6 weeks. Each week, 3 ml of the blood was drawn aseptically to measure hematological and serum biochemical parameters. Significant changes (P < 0.05) in packed cell volume (week 4), mean corpuscular volume (week 1 and 4), and total white blood cell count (week 3) were observed in CPDA-1 blood compared to CPD-SAGM. Glucose levels were higher in CPD-SAGM in weeks 5 and 6, while lactate dehydrogenase was significantly (P < 0.05) decreased. Aspartate aminotransferase showed significant increase in the CPDA-1 (P > 0.05) in weeks 7, 5, and 6. No significant difference was observed in the potassium concentration but sodium levels were higher in weeks 1 to 6 in the CPDA-1 blood bags. The result shows that hematological and serum biochemical changes were present in blood stored in CPDA-1 bags early compared to CPD-SAGM anticoagulant solution. In addition, blood stored in CPDA-1 bags may not maintain the cells viability for more than 35 days while blood can be stored for up to 42 days in the CPD-SAGM blood bags. Based on the findings, CPD-SAGM appeared to be a better blood storage solution than CPDA-1 in veterinary medicine due to it better glucose preservation, lower sodium, and lactate dehydrogenase concentrations which suggest reduced cellular damage.