<p>The presence of heavy elements in the serum of humans may serve as a reliable indicator for establishing the association between it with hyperbilirubinemia. This research aimed to investigate the impact of heavy metals such as chromium (Cr), cadmium (Cd), and lead (Pb) in the serum of Jaundice (hyperbilirubinemia) patients in infant and adult age groups. Sixty serum samples comprised 20 healthy individuals and 40 hyperbilirubinemia patients. The serum concentrations of Cr, Cd, and Pb were measured using atomic absorption spectroscopy (AAS) after sample preparation. The mean concentrations of these elements in the serum of male and female healthy individuals and patients for the two groups were calculated. The results showed a significant increase (<i>p</i> &lt; 0.05) in the Cd element for the infant group of males for patients compared with healthy groups. On the other hand, in adult groups, the results of Pb element for males, Cr, Cd, and Pb for females showed a significant increase (<i>p</i> &lt; 0.05) in patients compared with healthy groups. The results revealed that all heavy metals showed no significant difference (<i>p</i> &gt; 0.05) at different ages (infant and adult) of the patient groups. The results also revealed that heavy metals showed no significant difference (<i>p</i> &gt; 0.05) between males and females in patients, except for Cd in infants and Cr in adult patient groups. The statistical examination using the Statistical Package for the Social Sciences (SPSS) version 23 program disclosed no significant distinctions in the amounts of these substances among the two clusters. However, hyperbilirubinemia patients had higher Cr, Cd, and Pb concentrations than healthy individuals, indicating a possible association, and all values of Cr, Cd, and Pb concentrations in all serum samples were within the threshold limit value according to the American Conference of Governmental Industrial Hygienists (ACGIH).</p>