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<p>Centrifugal microfluidic platforms critically rely on valve units for precise fluid control. This paper introduces and investigates a novel centrifugal microfluidic valve that leverages capillary and centrifugal forces to accurately regulate fluid release at specific rotational speeds. The performance of the valve was experimentally evaluated under various conditions, confirming its capability for precise fluid handling within centrifugal systems. Next, as a practical application, we developed a microfluidic network incorporating these valves to automate the HbA1c testing process. The performance of the system was validated by automating test steps using dye-based water as a model fluid, achieving fluid transfer efficiencies exceeding 98% from inlet to reaction chambers across all stages. This automation significantly streamlines the workflow by eliminating time-consuming manual steps, reducing the need for specialized operators, and minimizing potential errors. We further confirmed that a minimum of five shake-mode mixing cycles (varying disk speed between 0 and 300&#xa0;rpm) achieves over 99% mixing efficiency within the reaction chamber, as quantified by Chroma analysis in the LAB color space. The simplicity of its fabrication process and the absence of large, expensive external equipment indicate that the system has the potential to be cost-effective and compatible with future point-of-care testing (POCT) devices, provided it is further validated with real assay reagents.</p>

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Design and evaluation of a capillary-centrifugal microfluidic valve for automated fluid handling

  • 期刊论文

  • 发表日期：

    2026年4月9日

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  • Mahan Kazemi,
  • Abas Ramiar,
  • Reza Derakhshan

摘要

Centrifugal microfluidic platforms critically rely on valve units for precise fluid control. This paper introduces and investigates a novel centrifugal microfluidic valve that leverages capillary and centrifugal forces to accurately regulate fluid release at specific rotational speeds. The performance of the valve was experimentally evaluated under various conditions, confirming its capability for precise fluid handling within centrifugal systems. Next, as a practical application, we developed a microfluidic network incorporating these valves to automate the HbA1c testing process. The performance of the system was validated by automating test steps using dye-based water as a model fluid, achieving fluid transfer efficiencies exceeding 98% from inlet to reaction chambers across all stages. This automation significantly streamlines the workflow by eliminating time-consuming manual steps, reducing the need for specialized operators, and minimizing potential errors. We further confirmed that a minimum of five shake-mode mixing cycles (varying disk speed between 0 and 300 rpm) achieves over 99% mixing efficiency within the reaction chamber, as quantified by Chroma analysis in the LAB color space. The simplicity of its fabrication process and the absence of large, expensive external equipment indicate that the system has the potential to be cost-effective and compatible with future point-of-care testing (POCT) devices, provided it is further validated with real assay reagents.