<p>Biosensors provide immense promise for detecting and assessing numerous anomalies in the human body. Lung cancer is the leading cause of cancer-related mortality worldwide. Introducing noninvasive diagnosis revolutionized the early detection of disease or abnormalities in medicine by capturing biomarkers. Saliva, perspiration, urea, bodily fluids, and exhaled breath are used for the bloodless diagnosis. The ideal system is composed of several volatile organic compounds (VOC) sensors, electronic signal conditioning circuits to extract signals from the sensors, a processing unit, and software for data analysis. This study reports the potential of glassy carbon electrode material prepared using carbon microelectromechanical systems (C-MEMS) with a TiO₂ sensing channel. The sensor was developed to detect acetone as a target molecule to diagnose abnormalities in the human body. This approach led to stable and reliable advances in the development of biosensors to detect VOC at room temperature and at an affordable cost. A sample 1 sensor is constructed combining a glassy carbon electrode with TiO₂ as the sensing material, which demonstrates a highly sensitive and consistent response to acetone vapors. The calculated response values of sample 1 is 31.85, whereas for sample 2 (constructed combining a glassy carbon electrode with TiO₂ and carbon nanotubes (CNTs)) is 22.78. There was a significant decrease from baseline resistance after exposure to acetone, indicating strong adsorption and charge transfer interactions with acetone molecules.</p>