Microfluidic separation device for blood components with lipids and cancer cells

In this paper, microfluidic chip with MCUV-channel is proposed for cancer cell separation of human blood with different components such as proteins/lipids, glucose and cancer cells. Existing hematoanalyzer instrument provides inaccurate cell separation results due to different components, viscosity levels and cells in human blood. The different blood components and cells in blood, reduces the hematoanalyzer accuracy. The proposed device is termed as MCUV device due to Multiple C-curves and U turns of V-shape. The proposed MCUV device consists of 5 distribution channels. The optimum range flow rate of MCUV is about 0.1–2.8 ml/min. In the proposed MCUV device, Multiple curves increases the secondary flow’s intensity and multiple bends at the end of every C turn enhances the pressure inside the microchannel, which helps for separation of the smaller particles such as platelets/plasma (< 2µm) from cancer cells(> 18µm). MCUV device has wider distribution channels, which increases the permeability. The proposed MCUV microfluidic chip is tested with Blood samples, which consists of cancer cells, without cancer cells and various viscosity levels. MCUV device separates multiple cells with an efficiency of > 85%. MCUV device tested for the performance of cell separation through Blood sample, which consists of proteins/lipids, glucose and cancer cells.