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<p>Light is a crucial environmental factor influencing plant growth and development. An efficient regeneration system is essential due to various limiting factors in the asexual reproduction of <i>Fraxinus mandshurica</i>. This study aimed to determine the optimal light conditions for the regeneration of adventitious buds in <i>F. mandshurica</i> using different red and blue light ratios. Using hypocotyls as explants, the most suitable medium for bud regeneration of <i>F. mandshurica</i> consisted of WPM supplemented with 1.0&#xa0;mg/L Thidiazuron (TDZ), 3.0&#xa0;mg/L 6-Benzylaminopurine (6-BA), 30&#xa0;g/L sucrose, and 7&#xa0;g/L agar (pH = 5.8-6.0). Monochromatic red light treatment effectively increased the adventitious bud induction rate to 2.25-fold of white light treatment, while the white light and B (blue): R (red) = 3:2 combination significantly promoted adventitious bud elongation. Combinations of light qualities at ratios of B: <i>R</i> = 2:3 and B: <i>R</i> = 1:4 significantly promoted root formation from seedling-derived shoots, increasing leaf area and chlorophyll content. Light conditions of B: <i>R</i> = 3:2 and B: <i>R</i> = 1:4 improved the chlorophyll a/b ratio, while a ratio of B: <i>R</i> = 4:1 promoted carotenoid synthesis. Furthermore, a correlation was observed between light quality and the expression of regeneration-related genes. The highest expression levels of <i>WUSHEL</i> (<i>FmWUS</i>), <i>WUSCHEL-relatedhomeobox</i> (<i>FmWOX</i>), and <i>WOUND INDUCED DEDIFFERENTIATION1 (FmWIND1)</i>, associated with regeneration, were recorded under red light (R). Similarly, the highest expression of elongation-related genes, <i>PHAVOLUTA</i> (<i>FmPHV</i>), <i>CUP-SHAPED COTYLEDON1 (FmCUC1)</i>, and <i>FmWOX4</i>, was found under blue: red (B: R) = 3:2. These gene expression results aligned with the observed phenotypes, suggesting that light quality plays a crucial role in bud regeneration and elongation. In summary, using and combining LED light sources resulted in significant improvements in the regeneration of adventitious shoots and adventitious roots for <i>F. mandshurica</i>.</p>

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Different light quality combinations promote adventitious shoot and adventitious root regeneration of Fraxinus mandshurica

  • 期刊论文

  • 发表日期：

    2026年1月12日

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  • Meihan Lin,
  • Shangzhu Gao,
  • Qiuju Jing,
  • Jiawei Yan,
  • Mengfan Zhao,
  • Yaguang Zhan,
  • Ying Xin,
  • Fansuo Zeng

摘要

Light is a crucial environmental factor influencing plant growth and development. An efficient regeneration system is essential due to various limiting factors in the asexual reproduction of Fraxinus mandshurica. This study aimed to determine the optimal light conditions for the regeneration of adventitious buds in F. mandshurica using different red and blue light ratios. Using hypocotyls as explants, the most suitable medium for bud regeneration of F. mandshurica consisted of WPM supplemented with 1.0 mg/L Thidiazuron (TDZ), 3.0 mg/L 6-Benzylaminopurine (6-BA), 30 g/L sucrose, and 7 g/L agar (pH = 5.8-6.0). Monochromatic red light treatment effectively increased the adventitious bud induction rate to 2.25-fold of white light treatment, while the white light and B (blue): R (red) = 3:2 combination significantly promoted adventitious bud elongation. Combinations of light qualities at ratios of B: R = 2:3 and B: R = 1:4 significantly promoted root formation from seedling-derived shoots, increasing leaf area and chlorophyll content. Light conditions of B: R = 3:2 and B: R = 1:4 improved the chlorophyll a/b ratio, while a ratio of B: R = 4:1 promoted carotenoid synthesis. Furthermore, a correlation was observed between light quality and the expression of regeneration-related genes. The highest expression levels of WUSHEL (FmWUS), WUSCHEL-relatedhomeobox (FmWOX), and WOUND INDUCED DEDIFFERENTIATION1 (FmWIND1), associated with regeneration, were recorded under red light (R). Similarly, the highest expression of elongation-related genes, PHAVOLUTA (FmPHV), CUP-SHAPED COTYLEDON1 (FmCUC1), and FmWOX4, was found under blue: red (B: R) = 3:2. These gene expression results aligned with the observed phenotypes, suggesting that light quality plays a crucial role in bud regeneration and elongation. In summary, using and combining LED light sources resulted in significant improvements in the regeneration of adventitious shoots and adventitious roots for F. mandshurica.