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<p>Biological pretreatment by white-rot fungi is a promising green technology for lignocellulose valorization, yet its industrial application is hampered by the high energy consumption of conventional steam sterilization. While a hurdle technology strategy combining low-temperature pasteurization and pH control has proven effective for wheat straw, its general applicability across diverse agricultural residues remains unclear. This study systematically compared the effects of low-temperature pasteurization (70&#xa0;°C and 80&#xa0;°C) with autoclaving (121&#xa0;°C) on the pretreatment of wheat, rice, and rapeseed straw by <i>Irpex lacteus</i> at an initial pH of 4.5. The results revealed significant substrate-specific responses. For wheat and rapeseed straw, pasteurization achieved enzymatic saccharification yields comparable to or exceeding those of autoclaving, demonstrating excellent energy-saving potential. In contrast, rice straw required autoclaving at 121&#xa0;°C to achieve maximum delignification (55.91% acid-insoluble lignin loss) and subsequent sugar release, likely due to its more recalcitrant native microbiota and structure. Pyrolysis-Gas Chromatography-Mass Spectrometry (Py-GC/MS) analysis confirmed that the S/G ratio decreased after pretreatment. Correlation analysis further established that the S/G ratio had a stronger negative correlation with glucose yield (<i>r</i> = − 0.94) than the total acid-insoluble lignin (AIL) content (<i>r</i> = − 0.90). These results suggest that the selective degradation of lignin structure, particularly the removal of S-units, is more closely associated with hydrolysis efficiency than total lignin removal. This work validates the viability of a low-temperature biological pretreatment strategy and underscores the necessity of tailoring protocols to specific feedstock characteristics to achieve efficient and economical biomass conversion.</p>

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Sterilization strategy determines degradation efficacy of diverse lignocellulosic biomasses by Irpex lacteus

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Ruling Yuan,
  • Qingbai Wu,
  • Jinping Li,
  • Dongze Niu,
  • Tingzhou Lei,
  • Taoli Huhe

摘要

Biological pretreatment by white-rot fungi is a promising green technology for lignocellulose valorization, yet its industrial application is hampered by the high energy consumption of conventional steam sterilization. While a hurdle technology strategy combining low-temperature pasteurization and pH control has proven effective for wheat straw, its general applicability across diverse agricultural residues remains unclear. This study systematically compared the effects of low-temperature pasteurization (70 °C and 80 °C) with autoclaving (121 °C) on the pretreatment of wheat, rice, and rapeseed straw by Irpex lacteus at an initial pH of 4.5. The results revealed significant substrate-specific responses. For wheat and rapeseed straw, pasteurization achieved enzymatic saccharification yields comparable to or exceeding those of autoclaving, demonstrating excellent energy-saving potential. In contrast, rice straw required autoclaving at 121 °C to achieve maximum delignification (55.91% acid-insoluble lignin loss) and subsequent sugar release, likely due to its more recalcitrant native microbiota and structure. Pyrolysis-Gas Chromatography-Mass Spectrometry (Py-GC/MS) analysis confirmed that the S/G ratio decreased after pretreatment. Correlation analysis further established that the S/G ratio had a stronger negative correlation with glucose yield (r = − 0.94) than the total acid-insoluble lignin (AIL) content (r = − 0.90). These results suggest that the selective degradation of lignin structure, particularly the removal of S-units, is more closely associated with hydrolysis efficiency than total lignin removal. This work validates the viability of a low-temperature biological pretreatment strategy and underscores the necessity of tailoring protocols to specific feedstock characteristics to achieve efficient and economical biomass conversion.