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<p>Bioaugmentation of anaerobic digestion (AD) systems is considered a cost-effective and environmentally friendly strategy to combat incomplete digestion of recalcitrant lignocellulosic substrates. This study investigated the lowest microbial inoculum size required for once-off bioaugmentation of AD cultures to enhance biomethane yield and process performance. The batch, laboratory-scale anaerobic co-digestion was carried out using pretreated corn stover (PCS) and food waste (FW), with cellulolytic <i>Bacillus subtilis, Serratia marcescens</i> and<i> Bacillus licheniformis.</i> The bioaugmentation screening was accomplished through a stepwise increase in the microbial loading using an initial standardised concentration of 0.4 × 10<sup>11</sup> colony-forming units (CFU)/mL within the system. Bioaugmentation decreased the digestion time by up to 11&#xa0;days. The inoculation of <i>B. subtilis</i> at a microbial concentration of 20 × 10<sup>11</sup>&#xa0;CFU/mL (4.85&#xa0;g DCW/L) improved the biomethane yield by 34% compared to the unaugmented control and produced 525 NmL CH<sub>4</sub>/gVS. Additionally, <i>S. marcescens</i> at 12 × 10<sup>11</sup>&#xa0;CFU/mL doubled the volumetric methane productivity from 0.47 ± 0.02 to 1.04 ± 0.02&#xa0;mL/(mL.day) when compared to the unaugmented control. The application of Nanopore sequencing after AD, to investigate the microbial community dynamics and structure in this treatment, underlined 43.52, 7.69 and 25.26% increases in the bacterial alpha diversity, namely the Shannon-, Simpson- and Observed indices, respectively. Moreover, a high abundance of between 50 and 80% of the <i>Firmicutes</i> population was identified.</p>

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Enhancing the biomethane production from lignocellulosic residues through bioaugmentation of anaerobic digestion

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月18日

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  • Jamie K. D. van Wyk,
  • Daneal C. S. Rorke,
  • Johann F. Gӧrgens,
  • Eugéne van Rensburg

摘要

Bioaugmentation of anaerobic digestion (AD) systems is considered a cost-effective and environmentally friendly strategy to combat incomplete digestion of recalcitrant lignocellulosic substrates. This study investigated the lowest microbial inoculum size required for once-off bioaugmentation of AD cultures to enhance biomethane yield and process performance. The batch, laboratory-scale anaerobic co-digestion was carried out using pretreated corn stover (PCS) and food waste (FW), with cellulolytic Bacillus subtilis, Serratia marcescens and Bacillus licheniformis. The bioaugmentation screening was accomplished through a stepwise increase in the microbial loading using an initial standardised concentration of 0.4 × 1011 colony-forming units (CFU)/mL within the system. Bioaugmentation decreased the digestion time by up to 11 days. The inoculation of B. subtilis at a microbial concentration of 20 × 1011 CFU/mL (4.85 g DCW/L) improved the biomethane yield by 34% compared to the unaugmented control and produced 525 NmL CH4/gVS. Additionally, S. marcescens at 12 × 1011 CFU/mL doubled the volumetric methane productivity from 0.47 ± 0.02 to 1.04 ± 0.02 mL/(mL.day) when compared to the unaugmented control. The application of Nanopore sequencing after AD, to investigate the microbial community dynamics and structure in this treatment, underlined 43.52, 7.69 and 25.26% increases in the bacterial alpha diversity, namely the Shannon-, Simpson- and Observed indices, respectively. Moreover, a high abundance of between 50 and 80% of the Firmicutes population was identified.