Scholarlink

<p>Prolonged exposure to pesticides is linked to neurodegenerative disorders through mechanisms involving oxidative stress, inflammation, and neuronal signaling. Therapeutic plants may offer a promising and natural alternative for protecting against such damage. Hence, the present study aims to understand the role of <i>Curcuma amada</i> in mitigating pesticide-induced neurotoxicity and its molecular mechanism in <i>Drosophila</i>. The pesticidal stress was induced in <i>Drosophila</i> through oral feed of ethion and its action was confirmed through behavioural assay. The stressed flies were treated with <i>C. amada</i> rhizome and the effect of both ethion and ethion +  <i>C. amada</i> was assessed through RNA profiling and gut microbiome analysis. Decrease in locomotory activity on exposure to ethion represents the induced neuronal stress and an increase was seen after <i>C. amada</i> was fed to the stressed flies. Many DEGs were identified through RNAseq results of stressed and <i>C. amada</i> treated which were further analysed using Cytoscape. In ethion and ethion + <i>C. amada</i> treated flies, the upregulated and downregulated genes were found to be associated with neuronal signal processing and mitochondrial function [MRPs, Dop2R, 5-HT1A, aminoacyl-tRNA synthetase (AARs), ND-B17]. A significant change in the gut microbial population (especially decrease in <i>Lactiplantibacillus</i> species) was observed in stressed flies. But the restoration of healthy bacterial population such as <i>Lactiplantibacillus</i> in <i>C. amada</i> treated flies evidencing the crucial role of gut microbiome in neuronal health. This study highlights the beneficial effects of <i>C. amada</i> from pesticidal stress which needs to be further researched to understand the underlying molecular mechanisms.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Neuroprotective role of Curcuma amada evidenced from pesticide-induced stressed Drosophila melanogaster: insights from RNAseq and gut microbiome analyses

  • 期刊论文

  • 发表日期：

    2026年5月2日

查看全文
  • Nikita Bindal,
  • Sujata Mohanty

摘要

Prolonged exposure to pesticides is linked to neurodegenerative disorders through mechanisms involving oxidative stress, inflammation, and neuronal signaling. Therapeutic plants may offer a promising and natural alternative for protecting against such damage. Hence, the present study aims to understand the role of Curcuma amada in mitigating pesticide-induced neurotoxicity and its molecular mechanism in Drosophila. The pesticidal stress was induced in Drosophila through oral feed of ethion and its action was confirmed through behavioural assay. The stressed flies were treated with C. amada rhizome and the effect of both ethion and ethion +  C. amada was assessed through RNA profiling and gut microbiome analysis. Decrease in locomotory activity on exposure to ethion represents the induced neuronal stress and an increase was seen after C. amada was fed to the stressed flies. Many DEGs were identified through RNAseq results of stressed and C. amada treated which were further analysed using Cytoscape. In ethion and ethion + C. amada treated flies, the upregulated and downregulated genes were found to be associated with neuronal signal processing and mitochondrial function [MRPs, Dop2R, 5-HT1A, aminoacyl-tRNA synthetase (AARs), ND-B17]. A significant change in the gut microbial population (especially decrease in Lactiplantibacillus species) was observed in stressed flies. But the restoration of healthy bacterial population such as Lactiplantibacillus in C. amada treated flies evidencing the crucial role of gut microbiome in neuronal health. This study highlights the beneficial effects of C. amada from pesticidal stress which needs to be further researched to understand the underlying molecular mechanisms.

Graphical abstract