Vector-borne pathogen evaluation and phylogenetic analysis in working K9 dogs in Iraq: implications for canine and public health
摘要
Canine (K9) units are essential in law enforcement and military operations; they are often at higher risk of vector-borne infections due to their working and kennel environments. This study aimed to investigate the prevalence of vector-borne pathogens, including Babesia sp. and Mycoplasma spp., in military/police K9 dogs which visited the Adan veterinary hospital in Baghdad/Iraq in 2016–2017. 61 blood samples were collected on Whatman FTA cards and sent to the University of Veterinary Medicine in Vienna, Austria where they were tested with specific PCRs to get a better understanding about epidemiological situation of these vector-borne pathogens in K9s in Iraq. Two dogs tested positive, one with Babesia sp. and one with Mycoplasma haemocanis, making the prevalence 1.6% (1/61; 95% CI: 0.04–8.8%) for each parasite, the isolates were sequenced, and phylogenetic analysis was done to confirm their lineage with other sequences from the GenBank. This is the first study on vector-borne pathogens in K9s in Iraq and the first molecular record of infection with M. haemocanis in K9s.