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<p>The present study provides a comprehensive analysis of the gut of the teleost fish <i>Trichopodus trichopterus</i>, with a focus on morphology, histology, histochemistry, and the localization of neuropeptides. Gross morphology revealed a well-delineated foregut, midgut, and hindgut. Histologically, HE staining confirmed the canonical arrangement of mucosa, submucosa, muscularis, and serosa. Histochemical assays such as periodic acid Schiff’s (PAS), Alcian blue (AB), and combined AB + PAS demonstrated region-specific distributions of neutral and acidic mucopolysaccharides within goblet cells. Immunohistochemical localization of neuropeptide Y (NPY) and secretagogin (SCGN) revealed a rich diversity of enteroendocrine cells (EECs) within the gut epithelium. NPY-immunoreactive EECs exhibited a wide variety of shapes and positions, including both open- and closed-type cells, with at least 16 distinct morphological variants. For the first time in lower vertebrates, we identified the presence of SCGN-immunoreactive cells, exhibiting notable diversity with approximately ten distinct cell types in gut. These findings suggest a complex and heterogeneous population of EECs involved in gut endocrine signaling and homeostasis in <i>T. trichopterus</i>. This study provides key insights into gut architecture and neuropeptide-secreting cell distribution in a teleost model, laying the groundwork for future research on EECs’ roles in nutrient absorption and intestinal homeostasis.</p>

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Morphology, histochemistry, and immunohistochemical characterization of neuropeptide Y and secretagogin in the gut of Trichopodus trichopterus

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Y. L. Gharad,
  • V. G. Barsagade,
  • S. A. Koushik,
  • K. T. Nakhate

摘要

The present study provides a comprehensive analysis of the gut of the teleost fish Trichopodus trichopterus, with a focus on morphology, histology, histochemistry, and the localization of neuropeptides. Gross morphology revealed a well-delineated foregut, midgut, and hindgut. Histologically, HE staining confirmed the canonical arrangement of mucosa, submucosa, muscularis, and serosa. Histochemical assays such as periodic acid Schiff’s (PAS), Alcian blue (AB), and combined AB + PAS demonstrated region-specific distributions of neutral and acidic mucopolysaccharides within goblet cells. Immunohistochemical localization of neuropeptide Y (NPY) and secretagogin (SCGN) revealed a rich diversity of enteroendocrine cells (EECs) within the gut epithelium. NPY-immunoreactive EECs exhibited a wide variety of shapes and positions, including both open- and closed-type cells, with at least 16 distinct morphological variants. For the first time in lower vertebrates, we identified the presence of SCGN-immunoreactive cells, exhibiting notable diversity with approximately ten distinct cell types in gut. These findings suggest a complex and heterogeneous population of EECs involved in gut endocrine signaling and homeostasis in T. trichopterus. This study provides key insights into gut architecture and neuropeptide-secreting cell distribution in a teleost model, laying the groundwork for future research on EECs’ roles in nutrient absorption and intestinal homeostasis.