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<p>Cytometric liver traits (e.g., cell area and nuclear-cytoplasmic ratio) are sensitive indicators of hepatic function, responding to diverse endogenous and exogenous factors. However, their application in population studies requires resolving two key methodological issues: how these traits vary across liver regions and the sample size that ensures reliable analysis. We analyzed hepatocyte density (cells per tissue unit area), a countable trait inversely correlated with cell area, but less labor-intensive to measure. In wild bank voles (<i>Clethrionomys glareolus</i> (Schreber, 1780)), we quantified density of mononuclear, binuclear, and anucleate hepatocytes across the liver lobes and two functionally distinct acinar zones (afferent/periportal vs. efferent/perivenous). The total number of cells analyzed (tens of thousands) exceeded by 6–7 times the minimum sample size (≈ 4,000) required to detect significant differences with an effect size as small as 2%, ensuring high statistical power. Using multinomial, binomial, and Poisson regression models (according to the type of data analyzed), we assessed the effects of sex, liver mass, and region on the density and proportion of the 3 types of hepatocytes. Hepatocyte density was consistently higher in efferent zones than in afferent zones (all cell types, 4% (95% CI: 3–5%)), but did not differ significantly between lobes or sexes. Liver mass significantly influenced hepatocyte composition; the proportion of anucleate cells increased proportionally with mass (log-odds = 0.53, <i>p</i> &lt; 0.001), suggesting that the anucleate/nuclear ratio could serve as a structural and/or functional marker. Our results suggest a link between the morphological and functional heterogeneity of hepatocytes and highlight the importance of considering the acinar zone when planning cytometric studies of the liver.</p>

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Assessing regional differences in hepatocyte density in wild rodents: a key step toward reliable liver cytometry

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Daria V. Poluektova,
  • Ivan A. Kshnyasev,
  • Yulia A. Davydova

摘要

Cytometric liver traits (e.g., cell area and nuclear-cytoplasmic ratio) are sensitive indicators of hepatic function, responding to diverse endogenous and exogenous factors. However, their application in population studies requires resolving two key methodological issues: how these traits vary across liver regions and the sample size that ensures reliable analysis. We analyzed hepatocyte density (cells per tissue unit area), a countable trait inversely correlated with cell area, but less labor-intensive to measure. In wild bank voles (Clethrionomys glareolus (Schreber, 1780)), we quantified density of mononuclear, binuclear, and anucleate hepatocytes across the liver lobes and two functionally distinct acinar zones (afferent/periportal vs. efferent/perivenous). The total number of cells analyzed (tens of thousands) exceeded by 6–7 times the minimum sample size (≈ 4,000) required to detect significant differences with an effect size as small as 2%, ensuring high statistical power. Using multinomial, binomial, and Poisson regression models (according to the type of data analyzed), we assessed the effects of sex, liver mass, and region on the density and proportion of the 3 types of hepatocytes. Hepatocyte density was consistently higher in efferent zones than in afferent zones (all cell types, 4% (95% CI: 3–5%)), but did not differ significantly between lobes or sexes. Liver mass significantly influenced hepatocyte composition; the proportion of anucleate cells increased proportionally with mass (log-odds = 0.53, p < 0.001), suggesting that the anucleate/nuclear ratio could serve as a structural and/or functional marker. Our results suggest a link between the morphological and functional heterogeneity of hepatocytes and highlight the importance of considering the acinar zone when planning cytometric studies of the liver.