Scholarlink

<p>We developed a threefold morphological protocol to maximize taxonomic data gathering for aquatic micrometazoans and applied it to <i>Dichaetura surreyi</i> Martin, 1990 in farm ditches in Northwest Germany (East Frisia) as proof of concept. To maximize morphological and anatomical information from the limited number of individuals, we applied a specimen-saving workflow that combines light microscopy, confocal laser scanning microscopy, and scanning electron microscopy on the same specimens. This approach refines the taxonomic understanding of <i>D. surreyi</i>, confirms originally described morphological features, and complements them with previously undescribed muscular architecture and cuticular characters for Dichaeturidae. We provide mitochondrial sequence data and a maximum-likelihood gene tree for Paucitubulatina placing D. <i>surreyi</i> as a potential sister to <i>Polymerurus</i>. The neotype designation and deposition of mitochondrial barcode sequences establish reference material for future research, and help address knowledge gaps in this poorly known family.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A threefold, specimen-saving morphological protocol for aquatic micrometazoans: proof of concept with an integrative re-investigation of the rare freshwater gastrotrich Dichaetura surreyi Martin, 1990

  • 期刊论文

  • 发表日期：

    2025年10月17日

查看全文
  • Axell Kou Minowa,
  • Thiago Quintão Araújo,
  • André Rinaldo Senna Garraffoni,
  • Alexander Kieneke

摘要

We developed a threefold morphological protocol to maximize taxonomic data gathering for aquatic micrometazoans and applied it to Dichaetura surreyi Martin, 1990 in farm ditches in Northwest Germany (East Frisia) as proof of concept. To maximize morphological and anatomical information from the limited number of individuals, we applied a specimen-saving workflow that combines light microscopy, confocal laser scanning microscopy, and scanning electron microscopy on the same specimens. This approach refines the taxonomic understanding of D. surreyi, confirms originally described morphological features, and complements them with previously undescribed muscular architecture and cuticular characters for Dichaeturidae. We provide mitochondrial sequence data and a maximum-likelihood gene tree for Paucitubulatina placing D. surreyi as a potential sister to Polymerurus. The neotype designation and deposition of mitochondrial barcode sequences establish reference material for future research, and help address knowledge gaps in this poorly known family.