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Aim <p>Early cervical cancer diagnosis is a global challenge that needs to be addressed by the discovery of less invasive diagnostic and prognostic approaches. Circulating miRNAs are stable in plasma and their diagnostic potentials have been elucidated in some cancers. Therefore, in this cross-sectional study, we determined the patterns of expression of 7 selected circulating microRNAs that differ between patients with cervical cancer receiving therapy, patients with cervical not on therapy and healthy females. The goal was to investigate the&#xa0;diagnostic and prognostic potential&#xa0;of these selected miRNAs.</p> Methods <p>Total RNA was extracted from plasma samples collected from&#xa0;53 participants&#xa0;recruited from Komfo Anokye Teaching Hospital and the Cape Coast Teaching Hospital, Ghana. Complementary DNA (cDNA) synthesis was performed, followed by quantitative polymerase chain reaction (qPCR) to amplify and quantify the expression levels of the target microRNAs. Expression levels of seven microRNAs—hsa-miR-146a, hsa-miR-29a, hsa-miR-29b, hsa-miR-34a, hsa-miR-233, hsa-miR-155, and hsa-miR-27a were compared among three groups: healthy controls (n = 27), patients with cervical cancer on therapy (n = 13), and those not on therapy (n = 13).</p> Results <p>miR-155 and miR-27a&#xa0;showed statistically significant differential expression between cancer patients and healthy controls. In addition,&#xa0;miR-29b&#xa0;expression levels differed significantly between&#xa0;stage 4b and stage 4a&#xa0;of patient with cervical cancer undergoing treatment.</p> Conclusion <p>These findings suggest that&#xa0;circulating plasma miRNAs&#xa0;may serve as&#xa0;non-invasive biomarkers&#xa0;for the early detection of cervical cancer, monitoring disease progression, and evaluating treatment response.</p>

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Expression patterns of plasma microRNAs in patients with cervical cancer from two teaching hospitals in Ghana

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月5日

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  • Helena Quayson,
  • Joseph Humphrey Kofi Bonney,
  • Daniel Sam,
  • Gloria Francisca Nuer-Allornuvor,
  • Roland Osei Saahene,
  • Nicholas Ekow Thomford,
  • Benjamin Amoani,
  • Francis Abrokwah,
  • Karen Pomeyie,
  • Musah Kalaamullah Salisu,
  • Prince Amoah Barnie

摘要

Aim

Early cervical cancer diagnosis is a global challenge that needs to be addressed by the discovery of less invasive diagnostic and prognostic approaches. Circulating miRNAs are stable in plasma and their diagnostic potentials have been elucidated in some cancers. Therefore, in this cross-sectional study, we determined the patterns of expression of 7 selected circulating microRNAs that differ between patients with cervical cancer receiving therapy, patients with cervical not on therapy and healthy females. The goal was to investigate the diagnostic and prognostic potential of these selected miRNAs.

Methods

Total RNA was extracted from plasma samples collected from 53 participants recruited from Komfo Anokye Teaching Hospital and the Cape Coast Teaching Hospital, Ghana. Complementary DNA (cDNA) synthesis was performed, followed by quantitative polymerase chain reaction (qPCR) to amplify and quantify the expression levels of the target microRNAs. Expression levels of seven microRNAs—hsa-miR-146a, hsa-miR-29a, hsa-miR-29b, hsa-miR-34a, hsa-miR-233, hsa-miR-155, and hsa-miR-27a were compared among three groups: healthy controls (n = 27), patients with cervical cancer on therapy (n = 13), and those not on therapy (n = 13).

Results

miR-155 and miR-27a showed statistically significant differential expression between cancer patients and healthy controls. In addition, miR-29b expression levels differed significantly between stage 4b and stage 4a of patient with cervical cancer undergoing treatment.

Conclusion

These findings suggest that circulating plasma miRNAs may serve as non-invasive biomarkers for the early detection of cervical cancer, monitoring disease progression, and evaluating treatment response.