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Background <p>Protein phosphatase 2 regulatory subunit A alpha (<i>PPP2R1A</i>) is the most common scaffold protein in the PP2A complex and has known tumor-suppressive functions. However, its role in gastric cancer (GC) is still unclear. This study aims to elucidate the potential regulatory role of <i>PPP2R1A</i> in the biological functions of GC.</p> Methods <p>The mutation status and expression levels of <i>PPP2R1A</i> in GC were assessed through bioinformatics analysis, the correlation between <i>PPP2R1A</i> levels and patient survival rates was examined, and its potential functional network was analyzed. Stable AGS and MGC803 cell lines were set up for overexpressing and silencing <i>PPP2R1A</i>. The effects on cell proliferation, migration, invasion, and apoptosis were assessed through CCK-8 assays, scratch assays, Transwell assays, and flow cytometry.</p> Results <p>The expression of <i>PPP2R1A</i> is significantly elevated in GC samples (<i>P</i> &lt; 0.001) and is not caused by mutations in <i>PPP2R1A</i> (<i>P</i> &gt; 0.05). Patients with high levels of <i>PPP2R1A</i> have a poorer 5-year survival rate (<i>P</i> &lt; 0.001). Silencing <i>PPP2R1A</i> significantly inhibits the proliferation, migration, and invasion of GC cells while promoting apoptosis (<i>P</i> &lt; 0.01). In contrast, overexpression of <i>PPP2R1A</i> does not have a significant impact on these cellular functions (<i>P</i> &gt; 0.05).</p> Conclusion <p><i>PPP2R1A</i> has potential oncogenic properties in the progression of GC, and knocking down the expression of <i>PPP2R1A</i> can inhibit the tumor progression of GC cells. This suggests that <i>PPP2R1A</i> may serve as a potential prognostic marker and therapeutic target for GC.</p>

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Silencing PPP2R1A inhibits the progression of gastric cancer cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月18日

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  • Gengming Cheng,
  • Laibijiang Wusiman,
  • Dingding Song,
  • Wenbin Zhang

摘要

Background

Protein phosphatase 2 regulatory subunit A alpha (PPP2R1A) is the most common scaffold protein in the PP2A complex and has known tumor-suppressive functions. However, its role in gastric cancer (GC) is still unclear. This study aims to elucidate the potential regulatory role of PPP2R1A in the biological functions of GC.

Methods

The mutation status and expression levels of PPP2R1A in GC were assessed through bioinformatics analysis, the correlation between PPP2R1A levels and patient survival rates was examined, and its potential functional network was analyzed. Stable AGS and MGC803 cell lines were set up for overexpressing and silencing PPP2R1A. The effects on cell proliferation, migration, invasion, and apoptosis were assessed through CCK-8 assays, scratch assays, Transwell assays, and flow cytometry.

Results

The expression of PPP2R1A is significantly elevated in GC samples (P < 0.001) and is not caused by mutations in PPP2R1A (P > 0.05). Patients with high levels of PPP2R1A have a poorer 5-year survival rate (P < 0.001). Silencing PPP2R1A significantly inhibits the proliferation, migration, and invasion of GC cells while promoting apoptosis (P < 0.01). In contrast, overexpression of PPP2R1A does not have a significant impact on these cellular functions (P > 0.05).

Conclusion

PPP2R1A has potential oncogenic properties in the progression of GC, and knocking down the expression of PPP2R1A can inhibit the tumor progression of GC cells. This suggests that PPP2R1A may serve as a potential prognostic marker and therapeutic target for GC.