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Background <p>Prostate cancer (PCa) is a prevalent malignancy within the male reproductive system that poses a significant threat to patients’ lives. The function of long non-coding RNA LINC01128 in PCa progression remains to be elucidated.</p> Objective <p>The objective was to evaluate the significance of LINC01128 in PCa and to elucidate the underlying mechanisms, thereby identifying a potential target for PCa treatment.</p> Methods <p>The clinical significance of LINC01128 in PCa was investigated by bioinformatics methods and data analysis. The expression of LINC01128 was quantified using real-time quantitative PCR. The impact of LINC01128 on PCa cell viability and metastasis was evaluated through Cell Counting Kit-8 and Transwell assays. The expression of epithelial-mesenchymal transition markers was analyzed by Western blot analysis. Bioinformatics methods and dual-luciferase reporter assay were employed to explore the mechanisms underlying the role of LINC01128 in PCa progression.</p> Results <p>LINC01128 demonstrated significant upregulation in PCa and exhibited a strong correlation with tumor-node-metastasis (TNM) stage, Gleason score, and lymph node metastasis. The upregulation of LINC01128 was found to be linked to a poorer prognosis for PCa. In PCa cells, silencing LINC01128 resulted in the suppression of cell proliferation, migration, and invasion. Furthermore, the knockdown of LINC01128 enhanced the expression of E-cadherin while concurrently repressing the expression of N-cadherin and Vimentin. Mechanistically, the negative regulation of miR-27b-3p by LINC01128 mediated the role of LINC01128 in PCa progression.</p> Conclusions <p>In PCa, high expression of LINC01128 may predict patients’ unfavorable prognosis. LINC01128 promoted PCa cellular processes by negatively regulating miR-27b-3p.</p>

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LncRNA LINC01128 promotes prostate cancer cell proliferation, metastasis, and epithelial-mesenchymal transition by modulating miR-27b-3p

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月4日

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  • Yuhui Zhao,
  • Zhihang Zhang,
  • Yi Zheng,
  • Huiming Bai,
  • Xiaotong Wu,
  • Yantao Yang,
  • Junfeng Zhang,
  • Chao Yu

摘要

Background

Prostate cancer (PCa) is a prevalent malignancy within the male reproductive system that poses a significant threat to patients’ lives. The function of long non-coding RNA LINC01128 in PCa progression remains to be elucidated.

Objective

The objective was to evaluate the significance of LINC01128 in PCa and to elucidate the underlying mechanisms, thereby identifying a potential target for PCa treatment.

Methods

The clinical significance of LINC01128 in PCa was investigated by bioinformatics methods and data analysis. The expression of LINC01128 was quantified using real-time quantitative PCR. The impact of LINC01128 on PCa cell viability and metastasis was evaluated through Cell Counting Kit-8 and Transwell assays. The expression of epithelial-mesenchymal transition markers was analyzed by Western blot analysis. Bioinformatics methods and dual-luciferase reporter assay were employed to explore the mechanisms underlying the role of LINC01128 in PCa progression.

Results

LINC01128 demonstrated significant upregulation in PCa and exhibited a strong correlation with tumor-node-metastasis (TNM) stage, Gleason score, and lymph node metastasis. The upregulation of LINC01128 was found to be linked to a poorer prognosis for PCa. In PCa cells, silencing LINC01128 resulted in the suppression of cell proliferation, migration, and invasion. Furthermore, the knockdown of LINC01128 enhanced the expression of E-cadherin while concurrently repressing the expression of N-cadherin and Vimentin. Mechanistically, the negative regulation of miR-27b-3p by LINC01128 mediated the role of LINC01128 in PCa progression.

Conclusions

In PCa, high expression of LINC01128 may predict patients’ unfavorable prognosis. LINC01128 promoted PCa cellular processes by negatively regulating miR-27b-3p.