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<p>Tissue macroarrays (Macro-TA), with 3–6&#xa0;mm tissue cores, are an alternative to Tissue Microarrays for small case cohorts, enhanced area selection, and spatial transcriptomics. Still, Macro-TA construction methods are time-consuming and have orientation challenges. We present a method for fast Macro-TA construction ensuring precise tissue orientation. Donor paraffin blocks matching HE slides were arranged using a layout for case identification. A punch biopsy was used to both perforate a sponge used as a scaffold and to extract tissue cores from donor paraffin blocks. Tissue cores were sequentially inserted into the sponge holes following the layout. Depending on the punch diameter, Macro-TAs contained 9 to 48 cores. Each Macro-TA block was constructed in 4 to 25&#xa0;min. This is a new, fast, easy, and reproducible method for Macro-TA construction without specialized instrumentation. It reduces 10× the processing time, ensuring proper sample orientation and being easy to implement for diagnostic and research pathology purposes.</p>

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An easy and reproducible method for rapid tissue macroarray block construction

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月20日

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  • Sandra Lopez-Prades,
  • Gabriela A. Caballero,
  • Iban Aldecoa,
  • Miriam Cuatrecasas

摘要

Tissue macroarrays (Macro-TA), with 3–6 mm tissue cores, are an alternative to Tissue Microarrays for small case cohorts, enhanced area selection, and spatial transcriptomics. Still, Macro-TA construction methods are time-consuming and have orientation challenges. We present a method for fast Macro-TA construction ensuring precise tissue orientation. Donor paraffin blocks matching HE slides were arranged using a layout for case identification. A punch biopsy was used to both perforate a sponge used as a scaffold and to extract tissue cores from donor paraffin blocks. Tissue cores were sequentially inserted into the sponge holes following the layout. Depending on the punch diameter, Macro-TAs contained 9 to 48 cores. Each Macro-TA block was constructed in 4 to 25 min. This is a new, fast, easy, and reproducible method for Macro-TA construction without specialized instrumentation. It reduces 10× the processing time, ensuring proper sample orientation and being easy to implement for diagnostic and research pathology purposes.