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<p>The morphological characteristics and cellular development of plant trichomes have been extensively studied, but research on their developmental mechanisms in medicinal plants such as <i>Lonicera japonica</i> Thunb (<i>L. japonica</i>) remains limited. Glandular trichomes (GTs) serve as crucial sites for flavonoid synthesis in <i>L. japonica</i>, and are closely associated with medicinal material quality. This study conducted transcriptome analysis on the trichomes and trichomeless flower buds of <i>L. japonica</i> “Sijihua.” Subsequently, two GT development-related genes, <i>LjHD8</i> and <i>LjMIXTA1</i>, were screened through homologous alignment. They were cloned, and the properties and structures of the corresponding proteins were analyzed. We also examined the expression patterns of the two genes and determined GT density on flower buds at various developmental stages. Moreover, subcellular localization revealed that the LjHD8 and LjMIXTA1 proteins were located in the cell nucleus. The genetic transformation results of <i>Nicotiana benthamiana</i> (<i>N. benth.</i>) indicated that the GT density of the two transgenic <i>N. benth.</i> lines was significantly higher than that of the wild-type. Additionally, HPLC analysis showed that isorhamnetin-3-O-glucoside (iso-3-O-glu) in both transgenic <i>N. benth.</i> lines was higher than wild-type. Therefore, <i>LjHD8</i> and <i>LjMIXTA1</i> play important roles in GT development and flavonoid compound synthesis in <i>L. japonica</i>. These findings contribute to understanding the molecular mechanism of trichome development in <i>L. japonica</i> and provide a basis for the evaluation of medicinal material quality, while also offering an important theoretical foundation for breeding new varieties with high GT density.</p>

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Cloning and functional verification of genes LjHD8 and LjMIXTA1 related to trichome development in Lonicera japonica Thunb.

  • 期刊论文

  • 发表日期：

    2026年6月29日

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  • Xinrui Fan,
  • Jing Fu,
  • Qiu Jiang,
  • Keyang Zhang,
  • Yongqing Zhang,
  • Lingna Wang

摘要

The morphological characteristics and cellular development of plant trichomes have been extensively studied, but research on their developmental mechanisms in medicinal plants such as Lonicera japonica Thunb (L. japonica) remains limited. Glandular trichomes (GTs) serve as crucial sites for flavonoid synthesis in L. japonica, and are closely associated with medicinal material quality. This study conducted transcriptome analysis on the trichomes and trichomeless flower buds of L. japonica “Sijihua.” Subsequently, two GT development-related genes, LjHD8 and LjMIXTA1, were screened through homologous alignment. They were cloned, and the properties and structures of the corresponding proteins were analyzed. We also examined the expression patterns of the two genes and determined GT density on flower buds at various developmental stages. Moreover, subcellular localization revealed that the LjHD8 and LjMIXTA1 proteins were located in the cell nucleus. The genetic transformation results of Nicotiana benthamiana (N. benth.) indicated that the GT density of the two transgenic N. benth. lines was significantly higher than that of the wild-type. Additionally, HPLC analysis showed that isorhamnetin-3-O-glucoside (iso-3-O-glu) in both transgenic N. benth. lines was higher than wild-type. Therefore, LjHD8 and LjMIXTA1 play important roles in GT development and flavonoid compound synthesis in L. japonica. These findings contribute to understanding the molecular mechanism of trichome development in L. japonica and provide a basis for the evaluation of medicinal material quality, while also offering an important theoretical foundation for breeding new varieties with high GT density.