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Main conclusion <p>Overexpression of <i>Chlorella ellipsoidea DGAT1</i> in soybean increases oil content and changes the components of fatty acids in seeds, resulting a great influence on lipid metabolism.</p> Abstract <p>High oil transgenic soybean germplasm is important for expanding soybean breeding resources. Here, <i>CeDGAT1</i>, a gene from <i>Chlorella ellipsoidea</i> encoded a key rate-limiting enzyme in triacylglycerol synthesis, was introduced into soybeans by <i>Agrobacterium</i>-mediated soybean cotyledon node method, and we have obtained an overexpression line <i>CeDGAT1</i>-OE#7 based on soybean cultivar Dengke12. Firstly, qRT-PCR analyses displayed that <i>CeDGAT1</i> was expressed in all the tissues of the transgenic line <i>CeDGAT1</i>-OE#7. And the total oil content was significantly higher by an average of 2.27% in seeds compared with the wild type. The analyses for the components of fatty acids showed that palmitic acid (16:0), stearic acids (18:0), and oleic acid (18:1) increased significantly by an average percentage of 1.34%, 0.62%, and 5.91%, respectively, while the contents of linoleic acid (18:2) and linolenic acids (18:3) were significantly lower than that of the WT. Furthermore, metabolomics analyses were conducted by using developing seeds of three stages, and a total of 1467, 1461, and 1078 significantly differentially expressed metabolites (DEMs) were separately identified. Two lipid-related pathways, linoleic acid metabolism and alpha-linolenic acid metabolism, were identified using KEGG pathway analysis of these DEMs, and seven metabolites in the linoleic acid metabolism pathway were down-regulated simultaneously, suggesting that these metabolites might be crucial in the response for overexpression of <i>CeDGAT1</i>. Together, our study has developed a high-oil transgenic soybean germplasm as a future breeding resource and those findings obtained from this research have broadened our understanding of the molecular mechanism underlying oil accumulation and fatty acid metabolism.</p>

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Overexpression of Chlorella ellipsoidea DGAT1 gene in soybean improves oil content and influences lipid metabolism

  • 期刊论文

  • 发表日期：

    2026年2月9日

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  • Hao Li,
  • Yueping Cao

摘要

Main conclusion

Overexpression of Chlorella ellipsoidea DGAT1 in soybean increases oil content and changes the components of fatty acids in seeds, resulting a great influence on lipid metabolism.

Abstract

High oil transgenic soybean germplasm is important for expanding soybean breeding resources. Here, CeDGAT1, a gene from Chlorella ellipsoidea encoded a key rate-limiting enzyme in triacylglycerol synthesis, was introduced into soybeans by Agrobacterium-mediated soybean cotyledon node method, and we have obtained an overexpression line CeDGAT1-OE#7 based on soybean cultivar Dengke12. Firstly, qRT-PCR analyses displayed that CeDGAT1 was expressed in all the tissues of the transgenic line CeDGAT1-OE#7. And the total oil content was significantly higher by an average of 2.27% in seeds compared with the wild type. The analyses for the components of fatty acids showed that palmitic acid (16:0), stearic acids (18:0), and oleic acid (18:1) increased significantly by an average percentage of 1.34%, 0.62%, and 5.91%, respectively, while the contents of linoleic acid (18:2) and linolenic acids (18:3) were significantly lower than that of the WT. Furthermore, metabolomics analyses were conducted by using developing seeds of three stages, and a total of 1467, 1461, and 1078 significantly differentially expressed metabolites (DEMs) were separately identified. Two lipid-related pathways, linoleic acid metabolism and alpha-linolenic acid metabolism, were identified using KEGG pathway analysis of these DEMs, and seven metabolites in the linoleic acid metabolism pathway were down-regulated simultaneously, suggesting that these metabolites might be crucial in the response for overexpression of CeDGAT1. Together, our study has developed a high-oil transgenic soybean germplasm as a future breeding resource and those findings obtained from this research have broadened our understanding of the molecular mechanism underlying oil accumulation and fatty acid metabolism.