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Main conclusion <p>PhC2H2-ZFP45 may act as a transcriptional activator to up-regulate the expression of <i>PhPAL2</i> and participate in the formation of floral fragrance.</p> Abstract <p>C2H2-zinc finger proteins (C2H2-ZFPs) are involved in the regulation of plant development and stress resistance. However, there are few studies on the effect of C2H2-ZFPs on the regulation of floral fragrance. <i>Petunia hybrida</i> has become an ideal model plant for studying floral volatile benzenoids/phenylpropanoids (FVBP) metabolism. To gain insight into the participation of C2H2-ZFPs in the regulation of floral fragrance in petunia, we performed a genome-wide identification and characterization of <i>C2H2-ZFP</i> genes. A total of 96 <i>C2H2-ZFP</i> genes were identified from the genome of <i>Petunia axillaris</i>, one wild parent of <i>P. hybrida</i>, and their gene structure, conserved motif, phylogenetic relationship, cis-acting elements were analyzed. The length and intron-exon organization in <i>P. axillaris C2H2-ZFP</i> genes were highly heterogeneous. The C2H2 domain was conserved in all C2H2-ZFPs, while the EAR domain was present in 33 C2H2-ZFPs. The <i>P. axillaris C2H2-ZFP</i> gene family was classified into four clades, and clade D contained 51 members. Most petunia <i>C2H2-ZFP</i> genes contained light stress response elements and hormone-related elements. 67 assembled sequences according to reported <i>P. hybrida</i> ‘Mitchell’ corolla RNA sequencing data could be mapped to the <i>C2H2-ZFP</i> genes of <i>Petunia axillaris</i>. The spatiotemporal expression patterns of <i>PhC2H2-ZFP8</i> and <i>PhC2H2-ZFP45</i> well correlated with the developmental and tissue-specific patterns of petunia floral scent formation and emission, suggesting that these genes might be involved in the regulation of FVBP metabolism. Through yeast one hybrid and dual luciferase assay, <i>PhC2H2-ZFP45</i> was further confirmed to upregulate the expression of <i>PhPAL2</i>. This study will serve as a molecular basis for further exploring the role of <i>PhC2H2-ZFPs</i> in floral scent regulation.</p>

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Genome-wide identification of petunia C2H2 zinc finger family genes and their potential roles in floral volatile benzenoids/phenylpropanoids metabolism

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Jiahong Chen,
  • Xiaomeng Liu,
  • Lingli Jiang,
  • Hongsheng Qing,
  • Jieyu Qian,
  • Zixi Li,
  • Chao Zhang,
  • Jianxin Fu

摘要

Main conclusion

PhC2H2-ZFP45 may act as a transcriptional activator to up-regulate the expression of PhPAL2 and participate in the formation of floral fragrance.

Abstract

C2H2-zinc finger proteins (C2H2-ZFPs) are involved in the regulation of plant development and stress resistance. However, there are few studies on the effect of C2H2-ZFPs on the regulation of floral fragrance. Petunia hybrida has become an ideal model plant for studying floral volatile benzenoids/phenylpropanoids (FVBP) metabolism. To gain insight into the participation of C2H2-ZFPs in the regulation of floral fragrance in petunia, we performed a genome-wide identification and characterization of C2H2-ZFP genes. A total of 96 C2H2-ZFP genes were identified from the genome of Petunia axillaris, one wild parent of P. hybrida, and their gene structure, conserved motif, phylogenetic relationship, cis-acting elements were analyzed. The length and intron-exon organization in P. axillaris C2H2-ZFP genes were highly heterogeneous. The C2H2 domain was conserved in all C2H2-ZFPs, while the EAR domain was present in 33 C2H2-ZFPs. The P. axillaris C2H2-ZFP gene family was classified into four clades, and clade D contained 51 members. Most petunia C2H2-ZFP genes contained light stress response elements and hormone-related elements. 67 assembled sequences according to reported P. hybrida ‘Mitchell’ corolla RNA sequencing data could be mapped to the C2H2-ZFP genes of Petunia axillaris. The spatiotemporal expression patterns of PhC2H2-ZFP8 and PhC2H2-ZFP45 well correlated with the developmental and tissue-specific patterns of petunia floral scent formation and emission, suggesting that these genes might be involved in the regulation of FVBP metabolism. Through yeast one hybrid and dual luciferase assay, PhC2H2-ZFP45 was further confirmed to upregulate the expression of PhPAL2. This study will serve as a molecular basis for further exploring the role of PhC2H2-ZFPs in floral scent regulation.