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Main conclusion <p><Emphasis Type="BoldItalic">TaHSFC3B</Emphasis> <b>enhanced the drought tolerance of overexpressed</b> <Emphasis Type="BoldItalic">Arabidopsis</Emphasis> <b>and decreased the drought tolerance of wheat silenced plants by participating in ROS scavenging and ABA pathway</b>.</p> Abstract <p>Heat shock transcription factors (HSFs) have extraordinary significance in plants' response to abiotic stress. But the specific function and mechanism of HSF in imparting drought resistance to wheat remain unclear. In this study, RT-qPCR and GUS staining suggested that the expression abundance of wheat HSF <i>TaHSFC3B</i> was high in grains, leaves, roots, and stems, and could be induced by PEG 6000 and abscisic acid (ABA). Subcellular localization displayed that the fluorescence signal of TaHSFC3B appeared in the nucleus, and transcriptional activation analysis indicated that full-length <i>TaHSFC3B</i> had no transcriptional activation activity. Overexpression of <i>TaHSFC3B</i> in <i>Arabidopsis</i> enhanced drought resistance by regulating the reactive oxygen species (ROS) and ABA pathways displaying improved antioxidant capacity, increased ABA accumulation and hypersensitivity, ultimately leading to reduced stomatal opening, higher leaf water content, elevated leaf temperature, and decreasing the survival rate under high temperature. In the transgenic <i>Arabidopsis</i> lines, the expression levels of genes associated with ROS and ABA pathways were significantly upregulated. In contrast, the silencing of <i>TaHSFC3B</i> in wheat resulted in a diminished antioxidant capacity and a reduced ABA accumulation, and subsequently led to reduced drought resistance, which specifically manifested as enlarged stomatal opening, increased leaf water loss, decreased temperature of detached leaves. <i>TaHSFC3B</i> silencing also significantly reduced the expression abundance of genes related to ROS and ABA pathways. This study provides important scientific support for a deeper understanding of the key functions of <i>HSF</i> and their potential applications in drought-resistant breeding, promoting the integration of research on plant stress tolerance mechanisms with practical breeding.</p>

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The wheat HSF transcription factor TaHSFC3B confers drought tolerance through ROS scavenging and ABA pathway in transgenic Arabidopsis and wheat (Triticum aestivum L.)

  • 期刊论文

  • 发表日期：

    2025年10月31日

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  • Yaning Bu,
  • Tianqi Song,
  • Jianfei Zhou,
  • Xinpeng Lei,
  • Xiling Chang,
  • Siyi Li,
  • Yuxin Wang,
  • Xiaoxing Wang,
  • Ling Kang,
  • Dongsheng Chen,
  • Jishan Xiang,
  • Xiaoke Zhang

摘要

Main conclusion

TaHSFC3B enhanced the drought tolerance of overexpressed Arabidopsis and decreased the drought tolerance of wheat silenced plants by participating in ROS scavenging and ABA pathway.

Abstract

Heat shock transcription factors (HSFs) have extraordinary significance in plants' response to abiotic stress. But the specific function and mechanism of HSF in imparting drought resistance to wheat remain unclear. In this study, RT-qPCR and GUS staining suggested that the expression abundance of wheat HSF TaHSFC3B was high in grains, leaves, roots, and stems, and could be induced by PEG 6000 and abscisic acid (ABA). Subcellular localization displayed that the fluorescence signal of TaHSFC3B appeared in the nucleus, and transcriptional activation analysis indicated that full-length TaHSFC3B had no transcriptional activation activity. Overexpression of TaHSFC3B in Arabidopsis enhanced drought resistance by regulating the reactive oxygen species (ROS) and ABA pathways displaying improved antioxidant capacity, increased ABA accumulation and hypersensitivity, ultimately leading to reduced stomatal opening, higher leaf water content, elevated leaf temperature, and decreasing the survival rate under high temperature. In the transgenic Arabidopsis lines, the expression levels of genes associated with ROS and ABA pathways were significantly upregulated. In contrast, the silencing of TaHSFC3B in wheat resulted in a diminished antioxidant capacity and a reduced ABA accumulation, and subsequently led to reduced drought resistance, which specifically manifested as enlarged stomatal opening, increased leaf water loss, decreased temperature of detached leaves. TaHSFC3B silencing also significantly reduced the expression abundance of genes related to ROS and ABA pathways. This study provides important scientific support for a deeper understanding of the key functions of HSF and their potential applications in drought-resistant breeding, promoting the integration of research on plant stress tolerance mechanisms with practical breeding.