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Main conclusion <p>This study identified 176 AP2/ERF genes in Solanum torvum, and investigated their sequence information. The functional analysis showed that StoERF109 positively functions in S. torvum against Verticillium wilt.</p> Abstract <p>Apetala2/ethylene-responsive factor (AP2/ERF) transcription factors play a crucial role in plant various biological processes including growth, development, phytohormone signaling, and stress responses. However, the sequence information, functions, and underlaying molecular mechanisms of <i>AP2/ERF</i> family members in <i>Solanum torvum</i>, a wild eggplant, largely remain unknown. Herein, a total of 176 <i>AP2/ERF</i> family genes in <i>S. torvum</i> were identified and classified into five sub-families: 23 <i>AP2 s</i>, 3 <i>RAVs</i>, 46 <i>DREBs</i>, 103 <i>ERFs</i>, and 1 <i>soloist</i>. The phylogenetic relationship, chromosomal assignment, collinearity, genes' structure, conserved domain, motif, and <i>cis</i>-element of <i>AP2/ERF</i> genes were investigated. An <i>ERF</i> sub-family gene <i>StoERF109</i> was identified, which expression was significantly up-regulated by <i>Verticillium dahliae</i> inoculation. Furthermore, a relative high expression of <i>StoERF109</i> was observed in stems of <i>S. torvum</i>. StoERF109 protein locates in the nucleus and exhibits transcriptional activation activity. Functional analysis indicated that silencing of <i>StoERF109</i> markedly enhanced susceptibility of <i>S. torvum</i> to Verticillium wilt, and significantly down-regulated the expression of defense-related genes including <i>StoABR1</i>, <i>StoAOS</i>, and <i>StoNDR1</i>. On the contrary, <i>StoERF109</i> transient expression up-regulated the expression of defense-related genes including <i>StoNPR1</i>, <i>StoEDS1</i>, <i>StoPAD4</i>, <i>StoNDR1</i>, <i>StoAOS</i>, and <i>StoABR1</i>. Our data provided insightful knowledge of the <i>AP2/ERF</i> family and revealed that <i>StoERF109</i> positively functions in <i>S. torvum</i> response to <i>V. dahliae</i> infection.</p>

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Genome-wide analysis of AP2/ERF gene family and functional characterization of StoERF109 in Solanum torvum response to Verticillium dahliae infection

  • 期刊论文

  • 发表日期：

    2025年5月20日

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  • Chang Feng,
  • Ya-lun Dong,
  • Lei Shen

摘要

Main conclusion

This study identified 176 AP2/ERF genes in Solanum torvum, and investigated their sequence information. The functional analysis showed that StoERF109 positively functions in S. torvum against Verticillium wilt.

Abstract

Apetala2/ethylene-responsive factor (AP2/ERF) transcription factors play a crucial role in plant various biological processes including growth, development, phytohormone signaling, and stress responses. However, the sequence information, functions, and underlaying molecular mechanisms of AP2/ERF family members in Solanum torvum, a wild eggplant, largely remain unknown. Herein, a total of 176 AP2/ERF family genes in S. torvum were identified and classified into five sub-families: 23 AP2 s, 3 RAVs, 46 DREBs, 103 ERFs, and 1 soloist. The phylogenetic relationship, chromosomal assignment, collinearity, genes' structure, conserved domain, motif, and cis-element of AP2/ERF genes were investigated. An ERF sub-family gene StoERF109 was identified, which expression was significantly up-regulated by Verticillium dahliae inoculation. Furthermore, a relative high expression of StoERF109 was observed in stems of S. torvum. StoERF109 protein locates in the nucleus and exhibits transcriptional activation activity. Functional analysis indicated that silencing of StoERF109 markedly enhanced susceptibility of S. torvum to Verticillium wilt, and significantly down-regulated the expression of defense-related genes including StoABR1, StoAOS, and StoNDR1. On the contrary, StoERF109 transient expression up-regulated the expression of defense-related genes including StoNPR1, StoEDS1, StoPAD4, StoNDR1, StoAOS, and StoABR1. Our data provided insightful knowledge of the AP2/ERF family and revealed that StoERF109 positively functions in S. torvum response to V. dahliae infection.