Introduction <p>Forensic professionals working in post-mortem centers are frequently exposed to traumatic events, making them susceptible to post-traumatic stress symptoms (PTSS) and related psychological distress. This study aimed to assess the prevalence and associated factors of PTSS among forensic medicine specialists, residents, autopsy technicians, and support staff in the Delhi-NCR region. </p> Methodology <p>A cross-sectional study was conducted across sixteen post-mortem centers, enrolling 184 participants equally divided into four occupational groups. Data were collected using a structured sociodemographic questionnaire and the Post-Traumatic Stress Symptoms Inventory (PTSSI). Statistical analyses were performed to identify associations between Post-Traumatic Stress Symptoms (PTSS) scores and sociodemographic as well as occupational factors. </p> Results <p>The mean age of participants was 38.5 years, with a male predominance (90.21%). The overall mean PTSS score was 9.68 (SD = 9.15), and only one participant met the criteria for PTSS. Higher PTSS scores were observed among autopsy technicians, involuntary workers, those with lower education, and participants who experienced fear during their first autopsy. Significant associations were found between PTSS scores and marital status, educational level, work attitude, workplace change requests, and negative family attitudes (<i>p</i> &lt; 0.05).</p> Discussion <p>Although the prevalence of clinically significant PTSS was low, certain subgroups-especially autopsy technicians and involuntary workers-exhibited elevated PTSS. Emotional distress was linked to specific work experiences and negative societal perceptions. Cultural resilience and family support may contribute to the overall low PTSS rates. Conclusion: Targeted mental health interventions, regular psychological screening, and supportive workplace policies are recommended to safeguard the well-being of forensic professionals exposed to occupational trauma.</p>