<p>Capillary electrophoresis (CE) is the conventional method for separating and detecting amplified DNA fragments for forensic human identification (HID). The Applied Biosystems^™ SeqStudio^™ Genetic Analyzer, referred to herein as the SeqStudio^™, is the most recent CE platform introduced for forensic purposes. The SeqStudio^™ includes an all-in-one reagent cartridge containing four capillaries. Validating a CE platform requires consideration of injection time, sensitivity and reproducibility, and mixture performance. The evaluation of the SeqStudio^™ was performed using a standard polymerase chain reaction (PCR)-CE workflow. Injection time, sensitivity, and mixture studies were performed to determine the optimal parameters for reproducible and reliable data. The injection time study yielded a general decline in the relative florescence units (RFU) as injection time decreased from ten to one second. The sensitivity study showed no locus or allelic dropout from one nanogram (ng) to 250 picogram (pg) DNA input at the one second injection time, however, when not considering the TH01 locus, the sensitivity was further reduced to 62.5pg. The two-person and three-person mixture studies demonstrated that the SeqStudio^™ is capable of detecting a large range of DNA mixture ratios. The evaluation serves as a model for best practices and supports the use of the SeqStudio^™ for HID.</p>