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Purpose <p>The popularity of electronic cigarettes (e-cigarettes) has grown exponentially over the past few years, and teenagers now prefer them to tobacco cigarettes. We determined whether exposure to e-cigarette vapour (e-vapour) adversely affects ion transport using human airway epithelial cell lines 16HBE14o- and Calu-3 and well-differentiated primary human bronchial epithelial cells (HBEs).</p> Methods <p>We concurrently measured fluorescent signals and short-circuit current (<i>I</i><sub><i>SC</i></sub>), an indicator of electrogenic ion transport, in polarised epithelia. The P2Y receptor-mediated signalling pathway was used to induce an increase in intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and <i>I</i><sub><i>SC</i></sub>. We used a single-polypeptide fluorescence resonance energy transfer reporter based on <Emphasis Type="Underline">e</Emphasis>xchange <Emphasis Type="Underline">p</Emphasis>roteins directly <Emphasis Type="Underline">a</Emphasis>ctivated by <Emphasis Type="Underline">c</Emphasis>AMP (Epac) to measure forskolin-induced changes in cAMP and <i>I</i><sub><i>SC</i></sub>.</p> Results <p>We compared the effects of e-vapour to those of traditional cigarette smoke (CS) on the human airway cell models. In all three cell types, e-vapour, similar to CS, significantly reduced agonist-induced increases in Ca<sup>2+</sup> or cAMP signalling and <i>I</i><sub><i>SC</i></sub>. However, reductions in the epithelial electrolyte transport activities did not correlate with any changes in the protein levels of various ion channels and transporters.</p> Conclusion <p>Our data suggest that e-vapour is not harmless and causes ion transport dysfunction similar to CS, thereby predisposing e-cigarette users to vaping-induced lung injury.</p>

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Effect of Electronic Cigarette Vapour Exposure on Ca2+- and cAMP-Dependent Ion Transport in Human Airway Epithelial Cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月18日

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  • Ya Niu,
  • Chung-Yin Yip,
  • Ke-wu Pan,
  • Judith Choi-Wo Mak,
  • Wing-Hung Ko

摘要

Purpose

The popularity of electronic cigarettes (e-cigarettes) has grown exponentially over the past few years, and teenagers now prefer them to tobacco cigarettes. We determined whether exposure to e-cigarette vapour (e-vapour) adversely affects ion transport using human airway epithelial cell lines 16HBE14o- and Calu-3 and well-differentiated primary human bronchial epithelial cells (HBEs).

Methods

We concurrently measured fluorescent signals and short-circuit current (ISC), an indicator of electrogenic ion transport, in polarised epithelia. The P2Y receptor-mediated signalling pathway was used to induce an increase in intracellular calcium concentration ([Ca2+]i) and ISC. We used a single-polypeptide fluorescence resonance energy transfer reporter based on exchange proteins directly activated by cAMP (Epac) to measure forskolin-induced changes in cAMP and ISC.

Results

We compared the effects of e-vapour to those of traditional cigarette smoke (CS) on the human airway cell models. In all three cell types, e-vapour, similar to CS, significantly reduced agonist-induced increases in Ca2+ or cAMP signalling and ISC. However, reductions in the epithelial electrolyte transport activities did not correlate with any changes in the protein levels of various ion channels and transporters.

Conclusion

Our data suggest that e-vapour is not harmless and causes ion transport dysfunction similar to CS, thereby predisposing e-cigarette users to vaping-induced lung injury.