Purpose <p>This study aimed to investigate the specific acoustic parameters to differentiate hypofunctional and hyperfunctional dysphonic groups, by comparing them with normophonic using multiparametric acoustic measures: Acoustic Voice Quality Index (AVQI v. 03.01), which objectively assess hoarseness and Acoustic Breathiness Index (ABI), which quantifies overall breathiness, along with their constituent parameters.</p> Methods <p>A total of 92 participants (aged 19–40) were included, divided into three groups: 46 normophonic individuals, 30 with hyperfunctional dysphonia, and 16 with hypofunctional dysphonia. AVQI and ABI values, along with their constituent parameters for sustained vowel <i>“sv”</i> and connected speech <i>“cs”</i> samples, were obtained using the <i>Praat</i> version 6.3.12.</p> Results <p>The results of One-way ANOVA followed by post hoc analysis revealed a significant difference for Smoothened Cepstral Peak Prominence (CPPS) was observed between hyperfunctional and hypofunctional groups; however, no significance was found in the mean AVQI value and its other constituent parameters. Further, a significant difference was observed for ABI values and their constituent parameters: GNE, HNR-D, and H₁H₂ across hyperfunctional and hypofunctional groups. In contrast, other ABI constituent parameters showed no significant differences.</p> Conclusions <p>The present study found that ABI and a few of its constituent parameters (GNE, HNR-D, H₁H₂) and CPPS (from AVQI) help in discriminating hypofunctional and hyperfunctional conditions significantly. These acoustic measures can complement auditory-perceptual assessment and contribute to more accurate clinical evaluation and diagnosis of voice disorders.</p>