<p>MicroRNAs (miRNAs) are recently among the emergent regulators in psoriasis. Psoriasis is one of the common autoimmune dermatological diseases that is characterized by inflammation, keratinocytes (KCs) hyperproliferation and defective differentiation. miRNA-195 is known to regulate proliferation and differentiation of cells in multiple diseases. In spite of this, its role in psoriasis pathogenesis is still vague. Overexpression of the multifunctional cytokine transforming growth factor-beta1 (TGFβ-1) in KCs can induce skin inflammation which contributes to the pathogenesis of psoriasis through a Smad-dependent mechanism. In this work, our aim was to detect the gene expression of miRNA-195 in psoriasis and its correlation with TGF-β1. Our study was a case-control one that involved 60 participants: 30 psoriasis vulgaris cases and 30 normal controls. To evaluate disease severity, Psoriasis Area and Severity Index (PASI), and the Body Surface Area (BSA) were used. The Dermatology Life Quality Index (DLQI) was used to assess Health-related quality of life (HRQOL). Serum miRNA-195 gene expression was evaluated by quantitative real-time polymerase chain reaction (qRT–PCR) and level of TGF-β1 was assessed by Enzyme-linked immunosorbent assay (ELISA). miRNA-195 gene expression showed significant decrease, while TGF-β1 showed a significant increase in cases as compared to controls. Gene expression of miRNA-195 did not show any significant correlations with the clinical parameters. There was a negative correlation between miRNA-195 gene expression and TGF‐β1 level (<i>p</i> &lt; 0.0001, <i>r</i> = − 0.915) in psoriasis. miRNA-195 gene expression in psoriasis is decreased, and it may participate in the disease pathogenesis. A possible relation between miRNA-195 and TGF-β1 may be suggested; however, further research is still needed to confirm this relationship.</p>