Scholarlink

<p>Atrial fibrillation (AF) is the most prevalent arrythmia worldwide and has a&#xa0;profound impact on patient’s quality of life. In cardiac surgery AF is a&#xa0;common occurrence. Roughly one in three patients undergoing cardiac surgery experiences an episode of AF; however, atrial remodelling which is of crucial relevance regarding the initiation and perpetuation of AF remains insufficiently understood. Many studies investigating the pathophysiology of AF focus on animal models or short-term experiments. Thus, establishing an experimental set-up for a&#xa0;long-term culture of human atrial myocardium was pursued. Following tissue procurement during open-heart surgery with cardiopulmonary bypass, trabeculae from the right atrial appendage were prepared and brought into organotypic culture for initially 12 and later also 21&#xa0;days under constant diastolic preload and electromechanical stimulation. During this time, contractile, electrophysiological and structural parameters as well as gene expression were assessed. No relevant differences between fresh and cultivated trabeculae were observed and the tissue viability at the end of culturing was also confirmed. The findings demonstrate that long-term cultivation of structurally intact, constantly beating human atrial myocardium is feasible. In the future, this model could be used to investigate the pathophysiology of atrial fibrillation as well as pharmacological effects directly in human atrial tissue.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Langzeitkultur von humanem Vorhofmyokard

  • 期刊论文

  • 发表日期：

    2026年5月11日

查看全文
  • Maximilian Klumm,
  • Thomas Seidel,
  • Christian Heim

摘要

Atrial fibrillation (AF) is the most prevalent arrythmia worldwide and has a profound impact on patient’s quality of life. In cardiac surgery AF is a common occurrence. Roughly one in three patients undergoing cardiac surgery experiences an episode of AF; however, atrial remodelling which is of crucial relevance regarding the initiation and perpetuation of AF remains insufficiently understood. Many studies investigating the pathophysiology of AF focus on animal models or short-term experiments. Thus, establishing an experimental set-up for a long-term culture of human atrial myocardium was pursued. Following tissue procurement during open-heart surgery with cardiopulmonary bypass, trabeculae from the right atrial appendage were prepared and brought into organotypic culture for initially 12 and later also 21 days under constant diastolic preload and electromechanical stimulation. During this time, contractile, electrophysiological and structural parameters as well as gene expression were assessed. No relevant differences between fresh and cultivated trabeculae were observed and the tissue viability at the end of culturing was also confirmed. The findings demonstrate that long-term cultivation of structurally intact, constantly beating human atrial myocardium is feasible. In the future, this model could be used to investigate the pathophysiology of atrial fibrillation as well as pharmacological effects directly in human atrial tissue.