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<p>The current research work aimed to develop, characterize, and assess in vitro cytotoxicity of resveratrol (RSV)-loaded lipid polymer hybrid nanocarriers (LPHN) in glioblastoma multiforme cells. RSV-LPHN was developed by central composite design (CCD). The developed RSV-LPHN were characterized by particle size (PS), polydispersity index (PDI), zeta (ζ) potential, % entrapment efficiency, and in vitro drug release. The surface morphology was studied using SEM and TEM techniques. FTIR, DSC, proton nuclear magnetic resonance (<sup>1</sup>H NMR) study, and X-ray diffraction studies (XRD) were also performed. The EE was found in the range of 62–70%, while PDI was between 0.126 and 0.264 exhibiting a monodispersed nature. Zeta potential was found between −15 and −46 mV. Surface morphology through TEM revealed smoothH needle-like structures of the RSV-LPHN. The optimized formulation exhibited nearly 87% drug release over 24 h revealing excellent sustained release behavior in comparison to pure drug. Excellent drug and excipient compatibility was observed in the FTIR study. DSC and <sup>1</sup>H NMR study revealed proper encapsulation of RSV in the lipidic matrix. XRD study confirmed the amorphous nature of the RSV-LPHN. Cellular uptake studies performed in U87 cells showed uptake of 162.26% from RSV-LPHN to control (99.38%) demonstrating maximum cellular internalization. Different concentrations of RSV showed a high percent of inhibition and anticancer activity against the glioblastoma cancer cell line as compared to 5FU. The present study authenticates the potential of RSV-LPHN for efficient management of glioblastoma multiforme.</p>

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Development, characterization, and in vitro cytotoxicity of resveratrol-loaded lipid polymer hybrid nanocarriers (LPHN) in glioblastoma multiforme cells

  • 期刊论文

  • 发表日期：

    2025年5月20日

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  • Swati Devendra Raysing,
  • Ashish Prakash Gorle

摘要

The current research work aimed to develop, characterize, and assess in vitro cytotoxicity of resveratrol (RSV)-loaded lipid polymer hybrid nanocarriers (LPHN) in glioblastoma multiforme cells. RSV-LPHN was developed by central composite design (CCD). The developed RSV-LPHN were characterized by particle size (PS), polydispersity index (PDI), zeta (ζ) potential, % entrapment efficiency, and in vitro drug release. The surface morphology was studied using SEM and TEM techniques. FTIR, DSC, proton nuclear magnetic resonance (1H NMR) study, and X-ray diffraction studies (XRD) were also performed. The EE was found in the range of 62–70%, while PDI was between 0.126 and 0.264 exhibiting a monodispersed nature. Zeta potential was found between −15 and −46 mV. Surface morphology through TEM revealed smoothH needle-like structures of the RSV-LPHN. The optimized formulation exhibited nearly 87% drug release over 24 h revealing excellent sustained release behavior in comparison to pure drug. Excellent drug and excipient compatibility was observed in the FTIR study. DSC and 1H NMR study revealed proper encapsulation of RSV in the lipidic matrix. XRD study confirmed the amorphous nature of the RSV-LPHN. Cellular uptake studies performed in U87 cells showed uptake of 162.26% from RSV-LPHN to control (99.38%) demonstrating maximum cellular internalization. Different concentrations of RSV showed a high percent of inhibition and anticancer activity against the glioblastoma cancer cell line as compared to 5FU. The present study authenticates the potential of RSV-LPHN for efficient management of glioblastoma multiforme.