Scholarlink

Background <p>Hepatoblastoma (HB) is the most common malignant liver tumor in pediatric patients. Even though the 5-year survival rate of HB patients has reached 80%, the prognosis in patients with high-risk features remain poor. This study aimed to establish the proteomic signature of HB to discover novel biomarkers and therapeutic targets.</p> Methods <p>HPLC-MS/MS coupled with bioinformatic analyses were performed to identify the differentially expressed proteins and related GO/KEGG pathways in HB tumor tissues. Molecular biology experiments in HepG2 and Huh6 cells were conducted to validate findings of proteomic results.</p> Results <p>Comparison of protein abundance profiles between HB tumors and normal adjacent liver tissues revealed high expression of HMGA2, IGFBP1, and LIN28B in HBs. GO/KEGG enrichment and protein–protein interaction analyses showed activation of RNA processing and the LIN28B/HMGA2 axis in HB tissue samples. Cellular and biochemical experiments confirmed that LIN28B expression was positively correlated with that of HMGA2, and that both promoted cell proliferation and tumor progression in the HB cell lines HepG2 and HUH6.</p> Conclusions <p>In this study, we mapped the protein abundance profile of HB, identified a possible regulatory role and therapeutic potential of the LIN28B/HMGA2 axis, and provided database and foundation for future studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Proteomic analysis reveals the pro-proliferative role of the LIN28B/HMGA2 axis in hepatoblastoma

  • 期刊论文

  • 发表日期：

    2026年3月12日

查看全文
  • Jia Shi,
  • Yangkun Wu,
  • Cheng Cheng,
  • Yeming Wu,
  • Zhixiang Wu

摘要

Background

Hepatoblastoma (HB) is the most common malignant liver tumor in pediatric patients. Even though the 5-year survival rate of HB patients has reached 80%, the prognosis in patients with high-risk features remain poor. This study aimed to establish the proteomic signature of HB to discover novel biomarkers and therapeutic targets.

Methods

HPLC-MS/MS coupled with bioinformatic analyses were performed to identify the differentially expressed proteins and related GO/KEGG pathways in HB tumor tissues. Molecular biology experiments in HepG2 and Huh6 cells were conducted to validate findings of proteomic results.

Results

Comparison of protein abundance profiles between HB tumors and normal adjacent liver tissues revealed high expression of HMGA2, IGFBP1, and LIN28B in HBs. GO/KEGG enrichment and protein–protein interaction analyses showed activation of RNA processing and the LIN28B/HMGA2 axis in HB tissue samples. Cellular and biochemical experiments confirmed that LIN28B expression was positively correlated with that of HMGA2, and that both promoted cell proliferation and tumor progression in the HB cell lines HepG2 and HUH6.

Conclusions

In this study, we mapped the protein abundance profile of HB, identified a possible regulatory role and therapeutic potential of the LIN28B/HMGA2 axis, and provided database and foundation for future studies.