Impact of hepatocyte growth factor on the colonic morphology and gut microbiome in short bowel syndrome rat model

期刊论文
发表日期：

2024年7月13日

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Koshiro Sugita,
Keisuke Yano,
Shun Onishi,
Yumiko Tabata,
Yumiko Iwamoto,
Masato Ogata,
Lynne Takada,
Chihiro Kedoin,
Masakazu Murakami,
Toshio Harumatsu,
Makoto Matsukubo,
Takafumi Kawano,
Mitsuru Muto,
Kotaro Kumagai,
Akio Ido,
Tatsuru Kaji,
Satoshi Ieiri

摘要

Purpose

This study aimed to investigate the impact of hepatocyte growth factor (HGF) on colonic morphology and gut microbiota in a rat model of short bowel syndrome (SBS).

Methods

SD rats underwent jugular vein catheterization for total parenteral nutrition (TPN) and 90% small bowel resection [TPN + SBS (control group) or TPN + SBS + intravenous HGF (0.3 mg/kg/day, HGF group)]. Rats were harvested on day 7. Colonic morphology, gut microflora, tight junction, and Toll-like receptor-4 (TLR4) were evaluated.

Results

No significant differences were observed in the colonic morphological assessment. No significant differences were observed in the expression of tight junction-related genes in the proximal colon. However, the claudin-1 expression tended to increase and the claudin-3 expression tended to decrease in the distal colon of the HGF group. The Verrucomicrobiota in the gut microflora of the colon tended to increase in the HGF group. The abundance of most LPS-producing microbiota was lower in the HGF group than in the control group. The gene expression of TLR4 was significantly downregulated in the distal colon of the HGF group.

Conclusion

HGF may enhance the mucus barrier through the tight junctions or gut microbiome in the distal colon.