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<p>Phenolic compounds regulate soil organic carbon (SOC) mineralization through inhibiting hydrolase activities, yet their effectiveness across varying soil pH and moisture conditions remains unclear. This study examined enzyme activity responses to catechol (0–100&#xa0;mg·kg<sup>−1</sup>) across a natural pH gradient (5.8–8.3) under contrasting moisture regimes. Our multi-enzyme approach revealed distinct pH thresholds governing phenolic compound effects: at pH &gt; 7, catechol significantly inhibited hydrolase activities by 5.45–13.14% regardless of moisture conditions, while at pH &lt; 7, it stimulated enzyme activities by 9.95–23.51%, particularly under flooded conditions. Short-term flooding fundamentally altered temporal inhibition patterns, with cellobiohydrolase activity showing a 10-day delayed response compared to aerobic conditions. Phenol oxidase exhibited contrasting responses, with catechol enhancing its activity under flooded conditions while suppressing it under non-flooded conditions. The flooding-induced microbial and enzymatic responses exhibited strong pH dependence, with greater effects at pH &gt; 7. Dissolved organic C dynamics displayed moisture-dependent biphasic patterns: decreasing then increasing under non-flooded conditions, while decreasing only at the later stage under flooded conditions. Structural equation modeling revealed that catechol indirectly inhibited hydrolase activities through microbial activity, subsequently affecting DOC content at pH &gt; 7. These findings extend enzymatic latch theory by demonstrating its pH dependency, with implications for C-related enzyme activities in agricultural systems experiencing variable moisture conditions.</p>

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pH thresholds govern the effects of catechol on some C-related enzyme activities under contrasting hydrological regimes

  • 期刊论文

  • 发表日期：

    2026年1月8日

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  • Peiqi Xin,
  • Zhenhua Chen,
  • Yulan Zhang,
  • Jian Gu,
  • Nan Jiang,
  • Lijun Chen

摘要

Phenolic compounds regulate soil organic carbon (SOC) mineralization through inhibiting hydrolase activities, yet their effectiveness across varying soil pH and moisture conditions remains unclear. This study examined enzyme activity responses to catechol (0–100 mg·kg−1) across a natural pH gradient (5.8–8.3) under contrasting moisture regimes. Our multi-enzyme approach revealed distinct pH thresholds governing phenolic compound effects: at pH > 7, catechol significantly inhibited hydrolase activities by 5.45–13.14% regardless of moisture conditions, while at pH < 7, it stimulated enzyme activities by 9.95–23.51%, particularly under flooded conditions. Short-term flooding fundamentally altered temporal inhibition patterns, with cellobiohydrolase activity showing a 10-day delayed response compared to aerobic conditions. Phenol oxidase exhibited contrasting responses, with catechol enhancing its activity under flooded conditions while suppressing it under non-flooded conditions. The flooding-induced microbial and enzymatic responses exhibited strong pH dependence, with greater effects at pH > 7. Dissolved organic C dynamics displayed moisture-dependent biphasic patterns: decreasing then increasing under non-flooded conditions, while decreasing only at the later stage under flooded conditions. Structural equation modeling revealed that catechol indirectly inhibited hydrolase activities through microbial activity, subsequently affecting DOC content at pH > 7. These findings extend enzymatic latch theory by demonstrating its pH dependency, with implications for C-related enzyme activities in agricultural systems experiencing variable moisture conditions.