Scholarlink

<p>Reductive soil disinfestation (RSD) has received widespread attention in the threat of antibiotic resistance in soil. However, the removal efficiency of this technology is still limited. To enhance the efficiency of the RSD technique, this study investigated the changes in antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and metal resistance genes (MRGs) during the application of biochar to soil via RSD. It also explored the influence of environmental factors and microbial communities on these genes. Results showed that compared to RCK (RSD without biochar), the relative abundance of ARGs in RC1 (1% biochar in RSD) and RC2 (2% biochar in RSD) was significantly reduced by 49.4% and 59.5% (<i>p</i> &lt; 0.05), respectively. Furthermore, compared to RCK, RC1 and RC2 demonstrated significant efficacy in reducing MGEs and MRGs, including <i>IS26</i>, <i>Tn916</i>, <i>merA-1</i>, and <i>czeA-1</i>, with RC2 being the most effective. Significant shifts occurred in the microbial community composition (<i>p</i> &lt; 0.05). Compared to RCK, The relative abundance of Proteobacteria and Actinobacteria was significantly reduced by 9.1% and 12.7%, respectively, in RC1 and RC2. The network analysis verified that the 35 bacterial genera showed significant positive correlations (<i>P</i> &lt; 0.05) with 12 different ARGs, MGEs, and MRGs. The phyla Proteobacteria and Actinobacteriota constituted the majority of these 35 bacterial genera and may be potential host bacteria for ARGs. Redundancy analysis (RDA) indicated that the reduction in ARGs was attributed to the decreased abundance of potential hosts. In conclusion, biochar enhanced the reduction of ARGs in soil by RSD and significantly reduced the relative abundance of ARGs, MGEs, and MRGs, with RC2 demonstrating superior efficacy to RC1. These findings provide valuable insights for developing sustainable agricultural practices and effectively reducing potential risks associated with antimicrobial resistance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Reducing antibiotic resistance genes through biochar-enhanced reductive soil disinfestation

  • 期刊论文

  • 发表日期：

    2025年10月22日

查看全文
  • Ranran Zhang,
  • Yuze Gao,
  • Qian Lou,
  • Chenpan Gong,
  • Yushui Chen,
  • Menglong Liu,
  • Yifei Zhang,
  • Changai Zhang,
  • Shengdao Shan,
  • Ming Hung Wong

摘要

Reductive soil disinfestation (RSD) has received widespread attention in the threat of antibiotic resistance in soil. However, the removal efficiency of this technology is still limited. To enhance the efficiency of the RSD technique, this study investigated the changes in antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and metal resistance genes (MRGs) during the application of biochar to soil via RSD. It also explored the influence of environmental factors and microbial communities on these genes. Results showed that compared to RCK (RSD without biochar), the relative abundance of ARGs in RC1 (1% biochar in RSD) and RC2 (2% biochar in RSD) was significantly reduced by 49.4% and 59.5% (p < 0.05), respectively. Furthermore, compared to RCK, RC1 and RC2 demonstrated significant efficacy in reducing MGEs and MRGs, including IS26, Tn916, merA-1, and czeA-1, with RC2 being the most effective. Significant shifts occurred in the microbial community composition (p < 0.05). Compared to RCK, The relative abundance of Proteobacteria and Actinobacteria was significantly reduced by 9.1% and 12.7%, respectively, in RC1 and RC2. The network analysis verified that the 35 bacterial genera showed significant positive correlations (P < 0.05) with 12 different ARGs, MGEs, and MRGs. The phyla Proteobacteria and Actinobacteriota constituted the majority of these 35 bacterial genera and may be potential host bacteria for ARGs. Redundancy analysis (RDA) indicated that the reduction in ARGs was attributed to the decreased abundance of potential hosts. In conclusion, biochar enhanced the reduction of ARGs in soil by RSD and significantly reduced the relative abundance of ARGs, MGEs, and MRGs, with RC2 demonstrating superior efficacy to RC1. These findings provide valuable insights for developing sustainable agricultural practices and effectively reducing potential risks associated with antimicrobial resistance.