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<p>To date, the mechanism of the effect of arbuscular mycorrhizal fungus (AMF) on the Cd uptake, transport and detoxification in <i>Solanum melongena</i> (eggplant) remain unclear. A pot experiment was performed to investigate the effect of AMF-<i>Glomus versiforme</i> (Gv) on the zinc (Zn) and phosphorus (P) uptake, rhizospheric soil characteristics, Cd accumulation, antioxidative activity and transcriptome in eggplant in the soils added with 0&#xa0;mg Cd kg<sup>− 1</sup>(Cd0) and 2&#xa0;mg Cd kg<sup>− 1</sup>(Cd2). In the Cd0 and Cd2 soils, Gv symbiosis significantly increased the photosynthesis, P/Zn uptake, soil phosphatase activity and growth of eggplant. Moreover, Gv colonization significantly increased the glomalin contents, pH, but decreased the effective Cd concentrations in rhizospheric soil, thus decreasing the Cd uptake in eggplant in the Cd2 soil. Additionally, Gv significantly improved the activities of the antioxidative enzymes and contents of antioxidative substances. More importantly in the Cd2 soil, transcriptome analysis showed that Gv symbiosis significantly upregulated <i>PT1</i>, <i>PT3</i> and <i>PT5</i> encoding phosphate transporter and <i>ZNE1</i> gene encoding Zn transporter, and thus improving the P/Zn absorption in eggplant. Furthermore, Gv colonization significantly downregulated <i>ZIP2</i>, <i>ZIP5</i> and <i>HMA2</i> and significantly upregulated <i>PCR6</i>, thus reducing the uptake, transport and accumulation of Cd in eggplant. In addition, <i>MT</i>, <i>ABCC2</i> and <i>MTP8</i> were evidently enhanced in mycorrhizal eggplant, which enhanced the chelation and compartmentalization of Cd and alleviated Cd toxicity in eggplant. The present study clarified the mechanism by which Gv symbiosis enhanced Zn/P acquisition and decreased the accumulation and toxicity of Cd in eggplant by a transcriptome analysis.</p>

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Transcriptome and Physiological Analyses Show that Arbuscular Mycorrhizal Fungus Promoted Growth and Reduced Cadmium Accumulation and Phytotoxification in Solanum melongena

  • 期刊论文

  • 发表日期：

    2026年7月2日

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  • Ren-Jie Li,
  • Pei-Xuan Xu,
  • Qi-Ying Zhu,
  • Yuan-Xiao Jing

摘要

To date, the mechanism of the effect of arbuscular mycorrhizal fungus (AMF) on the Cd uptake, transport and detoxification in Solanum melongena (eggplant) remain unclear. A pot experiment was performed to investigate the effect of AMF-Glomus versiforme (Gv) on the zinc (Zn) and phosphorus (P) uptake, rhizospheric soil characteristics, Cd accumulation, antioxidative activity and transcriptome in eggplant in the soils added with 0 mg Cd kg− 1(Cd0) and 2 mg Cd kg− 1(Cd2). In the Cd0 and Cd2 soils, Gv symbiosis significantly increased the photosynthesis, P/Zn uptake, soil phosphatase activity and growth of eggplant. Moreover, Gv colonization significantly increased the glomalin contents, pH, but decreased the effective Cd concentrations in rhizospheric soil, thus decreasing the Cd uptake in eggplant in the Cd2 soil. Additionally, Gv significantly improved the activities of the antioxidative enzymes and contents of antioxidative substances. More importantly in the Cd2 soil, transcriptome analysis showed that Gv symbiosis significantly upregulated PT1, PT3 and PT5 encoding phosphate transporter and ZNE1 gene encoding Zn transporter, and thus improving the P/Zn absorption in eggplant. Furthermore, Gv colonization significantly downregulated ZIP2, ZIP5 and HMA2 and significantly upregulated PCR6, thus reducing the uptake, transport and accumulation of Cd in eggplant. In addition, MT, ABCC2 and MTP8 were evidently enhanced in mycorrhizal eggplant, which enhanced the chelation and compartmentalization of Cd and alleviated Cd toxicity in eggplant. The present study clarified the mechanism by which Gv symbiosis enhanced Zn/P acquisition and decreased the accumulation and toxicity of Cd in eggplant by a transcriptome analysis.