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<p>Brassinosteroids (BRs) play crucial roles in plant growth and stress responses. However, the functions of BRs in leaf bending and root development remain largely unclear in wheat. In this study, BR treatment induced leaf bending and altered root architecture (RA), indicating that BRs exert a conserved role in regulating wheat plant architecture. Time-series analysis and weighted gene co-expression network analysis (WGCNA) identified three genes (<i>TaSLG-1A</i>, <i>TaSLG-1B</i>, and <i>TaSLG-1D</i>) as potential key regulators of leaf angle (LA) formation. Overexpression of <i>TaSLG-1A</i> in plants increased LA through asymmetric cell expansion on both sides of the lamina joint, accompanied by altered sensitivity to BR. Interaction network and promoter analyses suggested that <i>TaSLG-1A</i> is a potential target gene of two TabHLH62-like transcription factors. Furthermore, our results demonstrated that BRs modulate the accumulation and responses of auxin, gibberellin, and melatonin by regulating the expression of genes involved in their metabolism, which ultimately governs root growth. In addition, three auxin-related genes (<i>TaIAA61</i>, <i>TaARF9</i>, and <i>TaGH3-20</i>) and one melatonin biosynthetic gene (<i>TaM3H1/M3H2/M2H3-6</i>) were identified as potential candidates involved in RA regulation. Collectively, these findings provide important insights into the roles of BRs in wheat lamina joint development and root growth, and offer an effective strategy for improving wheat plant architecture through manipulating BR signaling pathways.</p>

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Transcriptome Analysis Reveals Core Regulatory Mechanisms Underlying Brassinosteroid-Mediated Leaf Inclination and Root Development in Wheat

  • 期刊论文

  • 发表日期：

    2026年6月26日

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  • Yingli Yang,
  • Hangkai Zhang,
  • Yinglei Li,
  • Jiaqi Zhou,
  • Liwei Zheng

摘要

Brassinosteroids (BRs) play crucial roles in plant growth and stress responses. However, the functions of BRs in leaf bending and root development remain largely unclear in wheat. In this study, BR treatment induced leaf bending and altered root architecture (RA), indicating that BRs exert a conserved role in regulating wheat plant architecture. Time-series analysis and weighted gene co-expression network analysis (WGCNA) identified three genes (TaSLG-1A, TaSLG-1B, and TaSLG-1D) as potential key regulators of leaf angle (LA) formation. Overexpression of TaSLG-1A in plants increased LA through asymmetric cell expansion on both sides of the lamina joint, accompanied by altered sensitivity to BR. Interaction network and promoter analyses suggested that TaSLG-1A is a potential target gene of two TabHLH62-like transcription factors. Furthermore, our results demonstrated that BRs modulate the accumulation and responses of auxin, gibberellin, and melatonin by regulating the expression of genes involved in their metabolism, which ultimately governs root growth. In addition, three auxin-related genes (TaIAA61, TaARF9, and TaGH3-20) and one melatonin biosynthetic gene (TaM3H1/M3H2/M2H3-6) were identified as potential candidates involved in RA regulation. Collectively, these findings provide important insights into the roles of BRs in wheat lamina joint development and root growth, and offer an effective strategy for improving wheat plant architecture through manipulating BR signaling pathways.