Unraveling the Potential Role of Light Emitting Diode and Substrate Type on Yield and Biochemical Characteristics of Sunflower Microgreens
摘要
Microgreens are increasingly recognized as functional foods due to their high phytochemical and nutrient density relative to mature plants. Their morphological development and biochemical composition are strongly regulated by environmental factors, particularly light spectrum and growing substrate. This study evaluated the interactive effects of substrate type and light quality on the growth, yield, and physiological responses of sunflower (Helianthus annuus L.) microgreens. A factorial experiment based on a completely randomized design with three replications was conducted under controlled environmental conditions. The two experimental factors were substrate type (soil vs. coco coir) and light spectrum (blue, red, white LED, and natural sunlight as control). Plants were harvested between 7 and 21 days post-sowing, depending on treatment. Measured parameters included fresh and dry yield, plant height, hypocotyl length, leaf area, nitrate concentration, peroxidase (POD) and ascorbate peroxidase (APX) activities, DPPH radical scavenging capacity, total phenolic content, and flavonoid concentration. Both substrate and light spectrum significantly influenced morphological and biochemical traits. The soil substrate combined with blue light produced the greatest plant height (134.8 mm), highest total phenolic content (0.466 mg g⁻¹ dry weight), and elevated flavonoid levels. Light spectrum significantly modulated antioxidant enzyme activity; notably, APX activity under natural sunlight was 65% higher than under all artificial light treatments. These findings demonstrate that strategic selection of substrate and light spectrum can effectively optimize both agronomic performance and nutritional quality in sunflower microgreens. Soil-based cultivation under blue light appears particularly advantageous for enhancing phenolic and flavonoid accumulation, while natural sunlight promotes stronger APX-mediated antioxidant defense.