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<p>The compound 7,8-dihydro-8α-20-hydroxyecdysone (DHECD) is a brassinosteroid (BR) mimic known to activate BR signaling and associated transcriptional responses. This study investigated the mechanism by which DHECD contributes to the maintenance of male fertility in rice under high-temperature (HT) stress. Rice plants at the panicle initiation stage were pretreated with DHECD prior to exposure to HT (40&#xa0;°C). HT stress disrupted pollen development and was associated with degeneration of the tapetal layer, resulting in abnormalities in pollen wall structure, reduced pollen viability, limited pollen tube growth on the stigma, and decreased seed set. DHECD pretreatment alleviated these defects and maintained relatively normal pollen development under HT conditions. Gene expression analysis showed that HT stress markedly increased the expression of tapetal programmed cell death (PCD)-related transcription factors (<i>OsTDR</i>, <i>OsTIP2</i>, and <i>OsEAT1</i>) and their downstream protease genes (<i>OsCP1</i>, <i>OsAP25</i>, and <i>OsAP37</i>). DHECD pretreatment reduced the HT-induced increase in the expression of these genes. In addition, DHECD-treated plants exhibited increased expression of the BR signaling transcription factor <i>OsBZR1</i> under HT conditions. Together, these results support a model in which DHECD, acting as a BR signaling agonist, influences the transcriptional network regulating tapetal PCD, thereby contributing to the maintenance of pollen development and male fertility under HT stress.</p>

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Exogenous Brassinosteroid Mimic Maintains Male Fertility Under High Temperature Through Regulation of Tapetum Programmed Cell Death Related Genes

  • 期刊论文

  • 发表日期：

    2026年4月17日

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  • Pantakarn Sukkho,
  • Natchaya Limkomolvilas,
  • Weerasin Sonjaroon,
  • Jianxin Shi,
  • Chatchawan Jantasuriyarat,
  • Kanapol Jutamanee

摘要

The compound 7,8-dihydro-8α-20-hydroxyecdysone (DHECD) is a brassinosteroid (BR) mimic known to activate BR signaling and associated transcriptional responses. This study investigated the mechanism by which DHECD contributes to the maintenance of male fertility in rice under high-temperature (HT) stress. Rice plants at the panicle initiation stage were pretreated with DHECD prior to exposure to HT (40 °C). HT stress disrupted pollen development and was associated with degeneration of the tapetal layer, resulting in abnormalities in pollen wall structure, reduced pollen viability, limited pollen tube growth on the stigma, and decreased seed set. DHECD pretreatment alleviated these defects and maintained relatively normal pollen development under HT conditions. Gene expression analysis showed that HT stress markedly increased the expression of tapetal programmed cell death (PCD)-related transcription factors (OsTDR, OsTIP2, and OsEAT1) and their downstream protease genes (OsCP1, OsAP25, and OsAP37). DHECD pretreatment reduced the HT-induced increase in the expression of these genes. In addition, DHECD-treated plants exhibited increased expression of the BR signaling transcription factor OsBZR1 under HT conditions. Together, these results support a model in which DHECD, acting as a BR signaling agonist, influences the transcriptional network regulating tapetal PCD, thereby contributing to the maintenance of pollen development and male fertility under HT stress.