<p>Soil salinization is an increasing threat to the cultivation of medicinal plants. This study evaluated the salt tolerance of four main <i>Andrographis paniculata</i> germplasms through multi-parametric comparison and membership function analysis under a range of salinity conditions in order to get a more comprehensive and accurate way for screening salt-tolerant germplasm resources. The result showed that (1) salt stress triggered negative growth responses across all the germplasms including reduction in plant height, leaf area, and biomass, while germplasm F showed the least reduction; (2) Meanwhile germplasm F demonstrated an escalation in antioxidant enzyme activities including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) as the salinity, contrasting with the diminished activities observed in other germplasms; (3) For photosynthesis, germplasm I and F showed an increase in chlorophyll content under salt stress, while germplasm A and H showed a decrease; (4) For secondary metabolites, andrographolide (AD), neoandrographolide (NAD) and 14-deoxyandrographolide (14-DAD) levels generally decrease as salinity increases, while dehydroandrographolide (DHAD) levels increase, except in germplasm F, where 14-DAD levels increase at low salinity. The membership function analysis positioned germplasm F as the most salt-tolerant, followed by H, I, and A. Morphological traits, antioxidant enzyme activities, proline (PRO), abscisic acid (ABA), and chlorophyll content were identified as reliable indicators for assessing salt tolerance. Germplasm F, with its superior salt tolerance, is a promising candidate for further research and cultivation.</p>