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<p>Phosphorus (P) mobilization via root-secreted organic acids, particularly citrate through metal chelation and proton-mediated acidification, is a key rhizosphere adaptation to P deficiency. Multidrug and Toxic Compound Extrusion (MATE) transporters emerge as critical regulators of citrate efflux. However, their roles in perennial fruit crops remain under explored. Here, we demonstrate root-specific induction of <i>MdMATE3</i> in apple under low-P conditions. Overexpressing <i>MdMATE3</i> enhanced citrate secretion and phosphorus uptake in both tobacco and apple systems. Transcriptomic and molecular analyses revealed that the low-P responsive transcription factor MdMYB1R directly activates <i>MdMATE3</i> by binding to the conserved TTATC motif of the promoter. This regulatory cascade enhances rhizosphere P availability through citrate-mediated solubilization and chelation, ultimately improving P uptake. This study elucidates <i>MdMATE3</i> pivotal role in low-P tolerance and provides molecular targets for developing P-efficient apple rootstocks.</p>

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Overexpression of MdMATE3 Promotes Citric Acid Secretion and Therefore Facilitates Phosphorus Uptake in Apple Plant

  • 期刊论文

  • 发表日期：

    2025年12月24日

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  • Kaiyu Wu,
  • Yuchen Zhang,
  • Xuewen Zhao,
  • Ting Wu,
  • Xuefeng Xu,
  • Zhenhai Han,
  • Changpeng Qiu

摘要

Phosphorus (P) mobilization via root-secreted organic acids, particularly citrate through metal chelation and proton-mediated acidification, is a key rhizosphere adaptation to P deficiency. Multidrug and Toxic Compound Extrusion (MATE) transporters emerge as critical regulators of citrate efflux. However, their roles in perennial fruit crops remain under explored. Here, we demonstrate root-specific induction of MdMATE3 in apple under low-P conditions. Overexpressing MdMATE3 enhanced citrate secretion and phosphorus uptake in both tobacco and apple systems. Transcriptomic and molecular analyses revealed that the low-P responsive transcription factor MdMYB1R directly activates MdMATE3 by binding to the conserved TTATC motif of the promoter. This regulatory cascade enhances rhizosphere P availability through citrate-mediated solubilization and chelation, ultimately improving P uptake. This study elucidates MdMATE3 pivotal role in low-P tolerance and provides molecular targets for developing P-efficient apple rootstocks.