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<p>Late Embryogenesis Abundant proteins (LEA) play critical roles in the adaptation of plants to various environmental constraints and especially dehydration. This study reports a genome-wide study of LEA proteins from barley (<i>Hordeum vulgare</i>). Indeed, 103 genes encoding for LEA family members were identified and classified into eight groups (LEA_1, LEA_2, LEA_3, LEA_4, LEA_5, LEA_6, DHN and SMP). Moreover, the LEA_2 group constitutes the largest one with 58 members while only one member belonging to LEA_6 group was identified. <i>HvLEA</i> genes were distributed in all chromosomes and harbour segmental and tandem duplications. Phylogenetic analyses allowed the classification of <i>HvLEA</i> proteins into eight phylogenetic groups and allowed the identification of putative orthologs in rice and Arabidopsis. Furthermore, in <i>silico</i> expression analyses allowed the identification of 52 stress-responsive <i>HvLEA</i> genes. The expression analysis of seven representative genes (<i>HvDHN6</i>, <i>HvDHN11</i>, <i>HvLEA1-6</i>, <i>HvLEA2-23</i>, <i>HvLEA3-2</i>, <i>HvLEA5-5</i> and <i>HvLEA6)</i> in two Tunisian barley cultivars (Manel and Rihane), under cold, salt and drought stresses, revealed that they were induced by all stress treatments with differential expression in the two genotypes. Moreover, four recombinant <i>Hv</i>LEA proteins (LEA 6, LEA2-23, DHN11 and LEA5-5) exhibited in vitro chaperone-like activities on Lactate Dehydrogenase (LDH) under cold, heat and desiccation treatments. These data underscore the genetic diversity of <i>HvLEA</i> genes and their responsiveness to abiotic stresses, illustrating their valuable role in enhancing barley’s resilience to adverse environmental conditions.</p>

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Genome-Wide Analysis of LEA Genes Family in Barley with Emphasis on their Role as Molecular Chaperones Under Abiotic Stress

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Ikram Zaidi,
  • Najeh Soltani,
  • Mohamed Najib Saidi

摘要

Late Embryogenesis Abundant proteins (LEA) play critical roles in the adaptation of plants to various environmental constraints and especially dehydration. This study reports a genome-wide study of LEA proteins from barley (Hordeum vulgare). Indeed, 103 genes encoding for LEA family members were identified and classified into eight groups (LEA_1, LEA_2, LEA_3, LEA_4, LEA_5, LEA_6, DHN and SMP). Moreover, the LEA_2 group constitutes the largest one with 58 members while only one member belonging to LEA_6 group was identified. HvLEA genes were distributed in all chromosomes and harbour segmental and tandem duplications. Phylogenetic analyses allowed the classification of HvLEA proteins into eight phylogenetic groups and allowed the identification of putative orthologs in rice and Arabidopsis. Furthermore, in silico expression analyses allowed the identification of 52 stress-responsive HvLEA genes. The expression analysis of seven representative genes (HvDHN6, HvDHN11, HvLEA1-6, HvLEA2-23, HvLEA3-2, HvLEA5-5 and HvLEA6) in two Tunisian barley cultivars (Manel and Rihane), under cold, salt and drought stresses, revealed that they were induced by all stress treatments with differential expression in the two genotypes. Moreover, four recombinant HvLEA proteins (LEA 6, LEA2-23, DHN11 and LEA5-5) exhibited in vitro chaperone-like activities on Lactate Dehydrogenase (LDH) under cold, heat and desiccation treatments. These data underscore the genetic diversity of HvLEA genes and their responsiveness to abiotic stresses, illustrating their valuable role in enhancing barley’s resilience to adverse environmental conditions.