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<p>Salinity stress severely affects the normal development, growth and yield of rice. Transcription factors (TFs) control a diverse array of gene; it is possible that they can influence salt stress responses via several mechanisms. In this study, for the first time, we report the functional characterization of one such salt responsive uncharacterized transcription factor <i>OsMADS16</i> of rice whose function is unknown under salinity. The <i>OsMADS16</i> overexpressing <i>Arabidopsis</i> lines have been found to show better tolerance during the germination, seedling and maturity stages under salinity stress compared to wild-type (WT) and <i>atmads16</i> SALK line (SALK_011532.56.00). Additionally, it was found that the overexpression of <i>OsMADS16</i> altered several physiological indices including an increase in proline, total sugar, chlorophyll contents, lateral root number as well as length of root and a decrease in malondialdehyde (MDA) content and the Na<sup>+</sup>/K<sup>+</sup> ratio that help plants to adapt adverse environmental condition. Our findings also revealed that SALK line exhibited salt-sensitive phenotype similar to WT plants after salinity stress treatment. Subcellular localization study revealed that the <i>OsMADS16</i> gene was localized in the nucleus of the cell. These findings suggest that the <i>OsMADS16</i> gene is involved in plant stress tolerance mechanism, that upregulate its expression level in order to enhance its ability to withstand salt stress in crop plants.</p>

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Heterologous Expression of OsMADS16 Transcription Factor of Rice Confers Salinity Stress Tolerance in Arabidopsis thaliana

  • 期刊论文

  • 发表日期：

    2025年11月3日

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  • Jyoti Nishad,
  • Soni Chowrasia,
  • Swati Mishra,
  • Alok Kumar Panda,
  • Nirmala Chongtham,
  • Tapan Kumar Mondal

摘要

Salinity stress severely affects the normal development, growth and yield of rice. Transcription factors (TFs) control a diverse array of gene; it is possible that they can influence salt stress responses via several mechanisms. In this study, for the first time, we report the functional characterization of one such salt responsive uncharacterized transcription factor OsMADS16 of rice whose function is unknown under salinity. The OsMADS16 overexpressing Arabidopsis lines have been found to show better tolerance during the germination, seedling and maturity stages under salinity stress compared to wild-type (WT) and atmads16 SALK line (SALK_011532.56.00). Additionally, it was found that the overexpression of OsMADS16 altered several physiological indices including an increase in proline, total sugar, chlorophyll contents, lateral root number as well as length of root and a decrease in malondialdehyde (MDA) content and the Na+/K+ ratio that help plants to adapt adverse environmental condition. Our findings also revealed that SALK line exhibited salt-sensitive phenotype similar to WT plants after salinity stress treatment. Subcellular localization study revealed that the OsMADS16 gene was localized in the nucleus of the cell. These findings suggest that the OsMADS16 gene is involved in plant stress tolerance mechanism, that upregulate its expression level in order to enhance its ability to withstand salt stress in crop plants.