Scholarlink

<p>Environmental stresses severely impact plant development and productivity by inducing the accumulation of reactive oxygen species (ROS). Peroxiredoxins (PRXs), cysteine-dependent peroxidases, serve as crucial ROS detoxifiers. Despite their importance, a comprehensive characterization of the <i>PRX gene</i> family in <i>Cicer arietinum</i> L. (chickpea) remains lacking. In this study, we identified nine <i>PRX genes</i> in the chickpea genome based on the presence of conserved domains (Redoxin, 1-Cys-PRX_C, AhpC-TSA) and a characteristic motif (PxxxTxxC—S–W/F). Phylogenetic comparisons with <i>Gossypium hirsutum</i>, <i>Oryza sativa</i>, and <i>Arabidopsis thaliana</i> revealed strong evolutionary conservation. Subcellular targeting analysis shows PRX proteins localized primarily to chloroplasts, mitochondria, and cytoplasm. Chromosomal mapping identifies their distribution across all seven chickpea chromosomes, with clusters on chromosomes 3 and 6. Gene structure analysis revealed 0–8 introns and 1–9 exons. Promoter analysis identified stress and hormone-responsive cis-elements, while miRNA target prediction suggests intricate post-transcriptional regulation. qRT-PCR validated differential expression of <i>PRX genes</i> under drought and salt stress conditions in chickpea. This genome-wide study provides foundational insights into PRX-mediated oxidative stress defense pathways, contributing to the understanding of chickpea adaptation to abiotic stress and offering valuable direction for developing stress-resilient cultivars through molecular breeding approaches.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide Identification and Expression Analysis of the Peroxiredoxin Gene from Chickpea (Cicer Arietinum L.) Unfold their Role Under Drought and Salt Stress Responses

  • 期刊论文

  • 发表日期：

    2025年11月3日

查看全文
  • Renu Kumari,
  • Mayur Patel,
  • Sanjib Kumar Panda

摘要

Environmental stresses severely impact plant development and productivity by inducing the accumulation of reactive oxygen species (ROS). Peroxiredoxins (PRXs), cysteine-dependent peroxidases, serve as crucial ROS detoxifiers. Despite their importance, a comprehensive characterization of the PRX gene family in Cicer arietinum L. (chickpea) remains lacking. In this study, we identified nine PRX genes in the chickpea genome based on the presence of conserved domains (Redoxin, 1-Cys-PRX_C, AhpC-TSA) and a characteristic motif (PxxxTxxC—S–W/F). Phylogenetic comparisons with Gossypium hirsutum, Oryza sativa, and Arabidopsis thaliana revealed strong evolutionary conservation. Subcellular targeting analysis shows PRX proteins localized primarily to chloroplasts, mitochondria, and cytoplasm. Chromosomal mapping identifies their distribution across all seven chickpea chromosomes, with clusters on chromosomes 3 and 6. Gene structure analysis revealed 0–8 introns and 1–9 exons. Promoter analysis identified stress and hormone-responsive cis-elements, while miRNA target prediction suggests intricate post-transcriptional regulation. qRT-PCR validated differential expression of PRX genes under drought and salt stress conditions in chickpea. This genome-wide study provides foundational insights into PRX-mediated oxidative stress defense pathways, contributing to the understanding of chickpea adaptation to abiotic stress and offering valuable direction for developing stress-resilient cultivars through molecular breeding approaches.