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<p>F-box proteins are a highly diverse group of proteins well-known for their pivotal roles in regulating protein degradation through the ubiquitin–proteasome pathway. These proteins are integral components of the Skp1-Cullin1-F-box E3 ubiquitin ligase complexes. The present study was aimed at deeper exploration of their structural and functional characteristics in pigeonpea. A set of 225 unique F-box gene members were identified and characterized. In-depth downstream <i>in-silico</i> analysis revealed the presence of 15 diverse functional domains within F-box proteins, including leucine-rich repeats, <i>Kelch</i> repeats, F-box-associated domains, domains of unknown function (DUF), and <i>Tubby</i> domains, among others. Furthermore, spatio-temporal gene expression analysis in contrasting cultivated genotypes, including a wild relative across different ovule/seed developmental stages revealed elevated expression of DUF, Transport Inhibitor Response 1, Lysine Motif, Phloem Protein 2, Heat Shock Protein 90, and Tryptophan-Aspartate Dipeptide (WD40) in the genotype with a high seed number per pod, suggesting their role in seed number regulation and, consequently, seed yield. Further, to understand the species-specific regulatory mechanism, more wild relatives can be included. The identified genes can be targeted for further functional validation through suitable gene editing approach.</p>

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Genomic Characterization of F-box Genes in Pigeonpea (Cajanus cajan (L.) Millspaugh): Insights into Evolution, Synteny, and Spatio-Temporal Expression During Ovule/Seed Development

  • 期刊论文

  • 发表日期：

    2025年10月10日

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  • Kumari Arpita,
  • Sandhya Sharma,
  • Arvind Kumar Yadav,
  • Machindra Nirgude,
  • Mohammad Israil Ansari,
  • Kishor Gaikwad

摘要

F-box proteins are a highly diverse group of proteins well-known for their pivotal roles in regulating protein degradation through the ubiquitin–proteasome pathway. These proteins are integral components of the Skp1-Cullin1-F-box E3 ubiquitin ligase complexes. The present study was aimed at deeper exploration of their structural and functional characteristics in pigeonpea. A set of 225 unique F-box gene members were identified and characterized. In-depth downstream in-silico analysis revealed the presence of 15 diverse functional domains within F-box proteins, including leucine-rich repeats, Kelch repeats, F-box-associated domains, domains of unknown function (DUF), and Tubby domains, among others. Furthermore, spatio-temporal gene expression analysis in contrasting cultivated genotypes, including a wild relative across different ovule/seed developmental stages revealed elevated expression of DUF, Transport Inhibitor Response 1, Lysine Motif, Phloem Protein 2, Heat Shock Protein 90, and Tryptophan-Aspartate Dipeptide (WD40) in the genotype with a high seed number per pod, suggesting their role in seed number regulation and, consequently, seed yield. Further, to understand the species-specific regulatory mechanism, more wild relatives can be included. The identified genes can be targeted for further functional validation through suitable gene editing approach.