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<p>The non-coding RNAs, particularly long non-coding RNAs (lncRNAs) are emerging regulators of plant development under stressful conditions. However, the systematic analysis of lncRNAs role during flower fertility-related heat stress responses has been elusive. We performed RNA-seq experiments at two flower bud developmental stages (S1, 1–2 mm; S2, 2–4 mm) in <i>Brassica napus</i> and identified 13,127 putative lncRNAs, of which 2640 and 3094 showed differential expression under heat stress. We also found that 73 lncRNAs were predicted to be endogenous target mimics of 23 miRNAs. Moreover, 32 lncRNAs were predicted as precursors of the 12 <i>B. napus</i> miRNAs family. Enrichment analysis of <i>cis</i>-/<i>trans</i>-regulated targets of lncRNAs indicated several important biological processes and pathways, including translation, metabolism, signal transduction, and spliceosome were enriched, suggesting their role in floral heat stress response. This study provides a systematic identification and characterization of lncRNAs involved in rapeseed flower development under heat stress, offering a foundational resource for future research in plant genomics, functional genetics, and evolutionary biology.</p>

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Heat-Responsive lncRNAome Landscape Associated with Flower Fertility Losses in Brassica napus

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Sana Basharat,
  • Shanshan Zhang,
  • Enerand Mackon,
  • Yun Li,
  • Pingwu Liu,
  • Muhammad Waseem

摘要

The non-coding RNAs, particularly long non-coding RNAs (lncRNAs) are emerging regulators of plant development under stressful conditions. However, the systematic analysis of lncRNAs role during flower fertility-related heat stress responses has been elusive. We performed RNA-seq experiments at two flower bud developmental stages (S1, 1–2 mm; S2, 2–4 mm) in Brassica napus and identified 13,127 putative lncRNAs, of which 2640 and 3094 showed differential expression under heat stress. We also found that 73 lncRNAs were predicted to be endogenous target mimics of 23 miRNAs. Moreover, 32 lncRNAs were predicted as precursors of the 12 B. napus miRNAs family. Enrichment analysis of cis-/trans-regulated targets of lncRNAs indicated several important biological processes and pathways, including translation, metabolism, signal transduction, and spliceosome were enriched, suggesting their role in floral heat stress response. This study provides a systematic identification and characterization of lncRNAs involved in rapeseed flower development under heat stress, offering a foundational resource for future research in plant genomics, functional genetics, and evolutionary biology.