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<p>Glycosyltransferases play a crucial role in catalyzing the conversion of aglycons into glycosylated conjugates, significantly impacting plant metabolism, stress adaptation, and secondary metabolite biosynthesis. This study investigates UDP-glycosyltransferase (UGT) diversity in <i>Glycyrrhiza glabra</i>, identifying 83 full-length <i>GgUGT</i> genes through transcriptome analysis. These genes were classified into 15 phylogenetic groups, including a novel Q group member previously unreported from legumes. Functional predictions were made by aligning each group with characterized proteins and protein–protein interactions. Conserved motifs, gene architecture, subcellular localization, physicochemical properties, and cis-acting regulatory elements were comprehensively analyzed. Expression profiling of differentially expressed genes indicated their roles in multiple stresses including pathogen resistance, drought tolerance, senescence, auxin, abscisic acid and methyl jasmonate. Notably, <i>GgUGT73B34</i> showed maximum transcript accumulation under drought and pathogen stress. Further, its promoter scanning confirmed multiple stress-responsive cis-acting elements for drought, pathogen, jasmonate signaling, auxin, and cytokinin-responsive motifs in upstream region. Heterologous transient expression of <i>Pro-GgUGT73B34</i> endorsed its activity under drought–pathogen–methyl jasmonate combined stress as assessed through GUS histochemical analysis and relative expression profiling. These findings enhance our understanding of the <i>GgUGTs</i> by illuminating its potential roles in plant stress responses, crop improvement, and secondary metabolite biosynthesis, while offering a solid foundation for future investigations into its biological functions.</p>

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Comprehensive Transcriptome-Wide Analysis of Differential UDP-Glycosyltransferases in the Stress Biology of Glycyrrhiza glabra

  • 期刊论文

  • 发表日期：

    2025年9月6日

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  • Bhawna Verma,
  • Shahnawaz Hussain,
  • Ritu Devi,
  • Palak Arora,
  • Rubeena Tabssum,
  • Fariha Chowdhary,
  • Suphla Gupta

摘要

Glycosyltransferases play a crucial role in catalyzing the conversion of aglycons into glycosylated conjugates, significantly impacting plant metabolism, stress adaptation, and secondary metabolite biosynthesis. This study investigates UDP-glycosyltransferase (UGT) diversity in Glycyrrhiza glabra, identifying 83 full-length GgUGT genes through transcriptome analysis. These genes were classified into 15 phylogenetic groups, including a novel Q group member previously unreported from legumes. Functional predictions were made by aligning each group with characterized proteins and protein–protein interactions. Conserved motifs, gene architecture, subcellular localization, physicochemical properties, and cis-acting regulatory elements were comprehensively analyzed. Expression profiling of differentially expressed genes indicated their roles in multiple stresses including pathogen resistance, drought tolerance, senescence, auxin, abscisic acid and methyl jasmonate. Notably, GgUGT73B34 showed maximum transcript accumulation under drought and pathogen stress. Further, its promoter scanning confirmed multiple stress-responsive cis-acting elements for drought, pathogen, jasmonate signaling, auxin, and cytokinin-responsive motifs in upstream region. Heterologous transient expression of Pro-GgUGT73B34 endorsed its activity under drought–pathogen–methyl jasmonate combined stress as assessed through GUS histochemical analysis and relative expression profiling. These findings enhance our understanding of the GgUGTs by illuminating its potential roles in plant stress responses, crop improvement, and secondary metabolite biosynthesis, while offering a solid foundation for future investigations into its biological functions.