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<p><i>Fusarium</i> wilt, caused by the soil-borne fungus <i>Fusarium oxysporum</i> f. sp. <i>ciceris</i> (FOC), poses a significant threat to global chickpea production. The traditional control tactics are often ineffective, necessitating searching for other, long-term strategies. Arbuscular mycorrhizal fungi (AMF) have emerged as potential biocontrol agents due to their ability to promote plant growth while guarding against disease. This study investigated the molecular interactions between chickpea, FOC, and AMF using RNA-seq technology. Chickpea plants were given three treatments: control, FOC-infected, and FOC + AMF co-inoculated. The biochemical parameters observed in the current experiment demonstrated decreased defense enzyme activity in FOC-treated plants, indicating a stress. However, AMF co-inoculation increased these enzyme activities, indicating an activation of defense strategy. Transcriptome analysis revealed distinct gene expression patterns among treatments. DEGs involved in the defense response, cell wall integrity, and stress response pathways were identified. Surprisingly, AMF co-inoculation altered these pathways, downregulating ethylene-mediated stress responses potentially increasing defense mechanisms. This study sheds light on the complex interplay between chickpea, FOC, and AMF at the molecular level and lays the door for creating long-term methods to combat <i>Fusarium</i> wilt by using AMF and hence, assuring food security.</p>

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Deciphering Chickpea Defense Strategies: Insights into Tripartite Interaction Between Chickpea, Fusarium oxysporum, and Arbuscular Mycorrhizal Fungi Treatments Using Transcriptomic and Biochemical Analysis

  • 期刊论文

  • 发表日期：

    2025年8月14日

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  • Samiksha Saluja,
  • Vinod Goyal,
  • Sarvjeet Kukreja

摘要

Fusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f. sp. ciceris (FOC), poses a significant threat to global chickpea production. The traditional control tactics are often ineffective, necessitating searching for other, long-term strategies. Arbuscular mycorrhizal fungi (AMF) have emerged as potential biocontrol agents due to their ability to promote plant growth while guarding against disease. This study investigated the molecular interactions between chickpea, FOC, and AMF using RNA-seq technology. Chickpea plants were given three treatments: control, FOC-infected, and FOC + AMF co-inoculated. The biochemical parameters observed in the current experiment demonstrated decreased defense enzyme activity in FOC-treated plants, indicating a stress. However, AMF co-inoculation increased these enzyme activities, indicating an activation of defense strategy. Transcriptome analysis revealed distinct gene expression patterns among treatments. DEGs involved in the defense response, cell wall integrity, and stress response pathways were identified. Surprisingly, AMF co-inoculation altered these pathways, downregulating ethylene-mediated stress responses potentially increasing defense mechanisms. This study sheds light on the complex interplay between chickpea, FOC, and AMF at the molecular level and lays the door for creating long-term methods to combat Fusarium wilt by using AMF and hence, assuring food security.